Simultaneous determination of (45)calcium and (65)zinc uptake by caco-2 cells.

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  • Additional Information
    • Source:
      Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print Cited Medium: Print ISSN: 0021-8561 (Print) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, American Chemical Society.
    • Subject Terms:
      Caco-2 Cells/*metabolism ; Calcium/*metabolism ; Scintillation Counting/*methods ; Zinc/*metabolism; Calcium Radioisotopes/metabolism ; Humans ; Zinc Radioisotopes/metabolism
    • Abstract:
      A simple method for simultaneously determining cell-associated Ca and Zn in Caco-2 cells is described. Calcium and zinc uptake was measured via radioisotopes (45)Ca and (65)Zn. Preliminary studies revealed that (65)Zn, a positron (beta(+)) and gamma emitter, contributed to (45)Ca counts in a liquid scintillation counter (LSC). However, (45)Ca, being a true beta emitter, did not contribute to the counts in a gamma counter (gammaC). To differentiate the counts of (45)Ca from those of (65)Zn, first a (65)Zn-labeled cell suspension was read in a gammaC and an LSC, thus obtaining the relationship between the radioactive counts obtained from the gammaC and LSC. This information defined the linear relationship between gammaC (65)Zn counts per minute (CPM) and LSC (65)Zn CPM. Because the (45)Ca and (65)Zn counts obtained in the LSC are additive, giving total LSC CPM, the value of LSC (45)Ca CPM was obtained by subtracting LSC (65)Zn CPM from total LSC CPM for the dual-labeled cell sample, obtaining then LSC (45)Ca CPM. To determine the absolute activity or disintegrations per minute (DPM) of each isotope in the dual-labeled sample, the linear relationship between DPM and CPM was determined for each isotope. The method is simple and straightforward for the determination of (45)Ca counts from a sample also containing (65)Zn, using gamma and liquid scintillation counters.
    • Accession Number:
      0 (Calcium Radioisotopes)
      0 (Zinc Radioisotopes)
      J41CSQ7QDS (Zinc)
      SY7Q814VUP (Calcium)
    • Publication Date:
      Date Created: 20021017 Date Completed: 20030130 Latest Revision: 20190723
    • Publication Date:
      20221213
    • Accession Number:
      10.1021/jf025706m
    • Accession Number:
      12381105