Recovery and separation of cell lysate proteins using hydrogels guided by aqueous two-phase extraction principles.

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  • Author(s): Putka CS;Putka CS; Gehrke SH; Willis M; Stafford D; Bryant J
  • Source:
    Biotechnology and bioengineering [Biotechnol Bioeng] 2002 Oct 20; Vol. 80 (2), pp. 139-43.
  • Publication Type:
    Comparative Study; Evaluation Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Wiley Country of Publication: United States NLM ID: 7502021 Publication Model: Print Cited Medium: Print ISSN: 0006-3592 (Print) Linking ISSN: 00063592 NLM ISO Abbreviation: Biotechnol Bioeng Subsets: MEDLINE
    • Publication Information:
      Publication: <2005->: Hoboken, NJ : Wiley
      Original Publication: New York, Wiley.
    • Subject Terms:
      Dextrans* ; Hydrogels* ; Polyethylene Glycols*; Chromatography, Gel/*methods ; Enzymes/*isolation & purification ; Proteins/*isolation & purification; Absorption ; Cell Fractionation ; Enzymes/biosynthesis ; Paracoccus/enzymology ; Solutions
    • Abstract:
      The addition of poly(ethylene glycol) and salts to clarified cell lysates of Thiosphaera pantotropha increases sorption of microbial proteins into dextran hydrogels, consistent with the thermodynamics of aqueous two-phase extraction. Addition of 12 wt% PEG-10,000 to the lysate increased total sorption of protein by the dextran gel from 5.2 mg/g dextran to 37 mg/g; addition of either 0.1 M potassium iodide or tetrabutylammonium fluoride along with PEG to the lysate increased protein sorption to more than 63 mg/g, a 12-fold increase. SDS-PAGE demonstrated that the type of salt added controls which proteins are absorbed by the gel. Previously demonstrated only with model solutions, these results suggest another approach to recovery and separation strategies for proteins produced by fermentation.
      (Copyright 2002 Wiley Periodicals, Inc.)
    • Accession Number:
      0 (Dextrans)
      0 (Enzymes)
      0 (Hydrogels)
      0 (Proteins)
      0 (Solutions)
      3WJQ0SDW1A (Polyethylene Glycols)
    • Publication Date:
      Date Created: 20020905 Date Completed: 20030312 Latest Revision: 20191210
    • Publication Date:
      20240829
    • Accession Number:
      10.1002/bit.10349
    • Accession Number:
      12209769