A sieving method for collecting the metacercariae of trematode parasites from freshwater fish.

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  • Author(s): Fan PC;Fan PC; Wu CC; Huang P; Yen CW
  • Source:
    The Southeast Asian journal of tropical medicine and public health [Southeast Asian J Trop Med Public Health] 2002 Mar; Vol. 33 (1), pp. 23-4.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Central Coordinating Board for Tropical Medicine and public Health Project of SEAMEC Country of Publication: Thailand NLM ID: 0266303 Publication Model: Print Cited Medium: Print ISSN: 0125-1562 (Print) Linking ISSN: 01251562 NLM ISO Abbreviation: Southeast Asian J Trop Med Public Health Subsets: MEDLINE
    • Publication Information:
      Original Publication: Bangkok, Thailand : Central Coordinating Board for Tropical Medicine and public Health Project of SEAMEC
    • Subject Terms:
      Fishes/*parasitology ; Parasite Egg Count/*methods ; Trematoda/*isolation & purification; Animals ; Centrifugation ; Fresh Water/parasitology ; Taiwan ; Trematoda/growth & development
    • Abstract:
      This study describes a sieving method for the collection of metacercariae from frozen (-20 degrees C) freshwater fish. Digested fish tissue is filtered through a series of sieves; the crude filtrate is then centrifuged. Centrifugation produces a sediment from which metacercariae can be removed. Half of the metracercariae that were obtained from the fish meat that had been frozen for 10 days (-20 degrees C) were dead; the other half were alive and some larvae were moving slowly.
    • Publication Date:
      Date Created: 20020718 Date Completed: 20021220 Latest Revision: 20061115
    • Publication Date:
      20240829
    • Accession Number:
      12118453