Rac1 和 WAVE2 蛋白在高脂饮食 C57BL/6J 幼鼠肾小球中的表达及意义.

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    • Alternate Title:
      Expression and significance of Rac1 and WAVE2 in glomerulus of high-fat diet induced C57BL/6J model mice.
    • Abstract:
      Objective To explore the expression and significance of Rac1 and WAVE2 protein in glomerulus of high-fat diet induced C57BL/6J model mice. Methods Thirty-two male C57BL/6J mice (3-week old) were randomly assigned into two groups(16 in each group). The control group was fed with basic diet (10% fat) for 4 weeks. The high-fat diet group was fed with high-fat diet (60% fat) for 4 weeks. The kidney morphological changes were examined by HE and PAS staining. The expressions of Rac1 and WAVE2 protein were examined by Western blot and immunohistochemistry analysis. Results HE and PAS results showed that there were glomeruli mesangial matrix hyperplasia and exudation in high- fat diet group compared with control group. The immunohistochemistry and Western blotting results showed that expressions of Rac1 and WAVE2 in glomerulus were both increased in high-fat diet group compared with those of control group. Conclusion Rac1 and WAVE2 protein may be involved in glomerular injuries induced by high-fat diet. [ABSTRACT FROM AUTHOR]
    • Abstract:
      目的探讨Rac1 和WAVE2 蛋白在高脂饮食诱导的C57BL/6J 幼鼠肾小球中的表达及意义。方法32 只3 周龄雄性C57BL/6J 幼鼠随机分为正常饮食组和高脂饮食组，每组16 只。分别给予标准饲料（脂肪含量10%） 与高脂饲料（脂肪含量60%）喂养4 周。HE 染色和PAS 染色观察小鼠肾脏的病理形态学改变；应用免疫组织化学技 术及蛋白免疫印迹技术检测Rac1 和WAVE2 蛋白的表达。结果与正常饮食组相比，高脂饮食喂养的C57BL/6J 幼 鼠出现了肾小球系膜基质轻度增生以及渗出等病理改变。同时高脂饮食组小鼠肾小球Rac1 和WAVE2 蛋白的表达 明显增强。结论Rac1 和WAVE2 蛋白可能共同参与了高脂饮食诱导的C57BL/6J 幼鼠肾小球的损伤。 [ABSTRACT FROM AUTHOR]
    • Abstract:
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