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Liquid chromatography/tandem mass spectrometric quantification with metabolite screening as a strategy to enhance the early drug discovery process.
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- Author(s): Tiller PR;Tiller PR; Romanyshyn LA
- Source:
Rapid communications in mass spectrometry : RCM [Rapid Commun Mass Spectrom] 2002; Vol. 16 (12), pp. 1225-31.
- Publication Type:
Journal Article
- Language:
English
- Additional Information
- Source:
Publisher: John Wiley And Sons Ltd Country of Publication: England NLM ID: 8802365 Publication Model: Print Cited Medium: Print ISSN: 0951-4198 (Print) Linking ISSN: 09514198 NLM ISO Abbreviation: Rapid Commun Mass Spectrom Subsets: MEDLINE
- Publication Information:
Publication: Chichester : John Wiley And Sons Ltd
Original Publication: London, UK : Heyden, c1987-
- Subject Terms:
- Abstract:
Throughput for early discovery drug metabolism studies can be increased with the concomitant acquisition of metabolite screening information and quantitative analysis using ultra-fast gradient chromatographic methods. Typical ultra-fast high-performance liquid chromatography (HPLC) parameters used during early discovery pharmacokinetic (PK) studies, for example, employ full-linear gradients over 1-2 min at very high flow rates (1.5-2 mL/min) on very short HPLC columns (2 x 20 mm). These conditions increase sample throughput by reducing analytical run time without sacrificing chromatographic integrity and may be used to analyze samples generated from a variety of in vitro and in vivo studies. This approach allows acquisition of more information about a lead candidate while maintaining rapid analytical turn-around time. Some examples of this approach are discussed in further detail.
(Copyright 2002 John Wiley & Sons, Ltd.)
- Accession Number:
0 (Cardiovascular Agents)
0 (Glucuronides)
0 (Hypoglycemic Agents)
- Publication Date:
Date Created: 20020712 Date Completed: 20020812 Latest Revision: 20041117
- Publication Date:
20231215
- Accession Number:
10.1002/rcm.708
- Accession Number:
12112275
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