Nephrin expression in the post-natal developing kidney in normotensive and hypertensive rats.

Publisher: Informa Healthcare Country of Publication: England NLM ID: 9305929 Publication Model: Print Cited Medium: Print ISSN: 1064-1963 (Print) Linking ISSN: 10641963 NLM ISO Abbreviation: Clin Exp Hypertens Subsets: MEDLINE

Publication: London : Informa Healthcare
Original Publication: New York, N.Y. : Marcel Dekker, Inc., c1993-

Gene Expression Regulation, Developmental*; Hypertension/*physiopathology ; Kidney/*physiology ; Proteins/*genetics; Albuminuria/physiopathology ; Animals ; Blood Pressure ; In Situ Hybridization ; Kidney/chemistry ; Kidney/growth & development ; Male ; Membrane Proteins ; Proteins/analysis ; RNA, Messenger/analysis ; Rats ; Rats, Inbred SHR ; Rats, Inbred WKY

Nephrin is a slit diaphragm protein and its expression in the developing kidney is largely unknown. In this study, we explored the expression of nephrin in the developmental kidney in spontaneously hypertensive (SHR) and in Wistar-Kyoto (WKY) rats at different time points, from day 5 after birth to adulthood. Real time RT-PCR, in situ hybridization and immunohistochemistry were used to assess and quantify gene and protein expression of nephrin in the kidney. SHR had hypertension at week 10 and albuminuria at week 20. Nephrin expression in both SHR and WKY increased from day 5 to adulthood. Furthermore, both gene and protein expression of nephrin were significantly lower in SHR after birth when compared to WKY at the same age. These findings suggest that both in normotensive and hypertensive rats, nephrin expression increased from birth to the adult age and that down-regulation of nephrin in SHR evident from the early developmental kidney to adulthood may contribute to the development of albuminuria in adult SHR.

0 (Membrane Proteins)
0 (Proteins)
0 (RNA, Messenger)
0 (nephrin)

Date Created: 20020712 Date Completed: 20030113 Latest Revision: 20191106

20240627

10.1081/ceh-120004798

12109777