Connexin 43 suppresses human glioblastoma cell growth by down-regulation of monocyte chemotactic protein 1, as discovered using protein array technology.

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  • Additional Information
    • Source:
      Publisher: American Association for Cancer Research Country of Publication: United States NLM ID: 2984705R Publication Model: Print Cited Medium: Print ISSN: 0008-5472 (Print) Linking ISSN: 00085472 NLM ISO Abbreviation: Cancer Res Subsets: MEDLINE
    • Publication Information:
      Publication: Baltimore, Md. : American Association for Cancer Research
      Original Publication: Chicago [etc.]
    • Subject Terms:
      Chemokine CCL2/*biosynthesis ; Connexin 43/*physiology ; Glioblastoma/*metabolism ; Glioblastoma/*pathology; Cell Division/physiology ; Chemokine CCL2/genetics ; Connexin 43/genetics ; Cytokines/biosynthesis ; Cytokines/genetics ; Down-Regulation ; Gene Expression Profiling ; Glioblastoma/genetics ; Humans ; Oligonucleotide Array Sequence Analysis ; Transfection
    • Abstract:
      Previously, we demonstrated that connexin 43 (cx43) suppressed the growth of human glioblastoma cells. To investigate the molecular mechanisms involved in tumor suppression by cx43, we developed a human cytokine array system, which simultaneously detects the expression of 43 cytokines. By using this new technology, we analyzed the cx43-regulated genes in cx43-transfected cells. The cytokine arrays showed that expression of monocyte chemotactic protein-1 (MCP-1) was profoundly reduced in cx43-transfected cells. RT-PCR, immuno-Western blot, and cDNA microarrays further confirmed this observation. Addition of conditioned medium from control-transfected cells and recombinant MCP-1 to cx43-transfected cells significantly enhanced cx43-transfected cell proliferation and colony formation in soft agar. In contrast, addition of neutralization antibody against MCP-1 significantly inhibited cell proliferation in control-transfected cells. Our results suggested that MCP-1 is involved in the suppression of human glioblastoma cell growth by cx43.
    • Grant Information:
      CA 39745 United States CA NCI NIH HHS; CA 89273 United States CA NCI NIH HHS
    • Accession Number:
      0 (Chemokine CCL2)
      0 (Connexin 43)
      0 (Cytokines)
    • Publication Date:
      Date Created: 20020523 Date Completed: 20020710 Latest Revision: 20071114
    • Publication Date:
      20221213
    • Accession Number:
      12019157