Genetic and biochemical characterization of the broad spectrum chlorobenzene dioxygenase from Burkholderia sp. strain PS12: Dechlorination of 1,2,4,5-tetrachlorobenzene.

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    • Subject Terms:
      CHLOROBENZENE; DNA; ESCHERICHIA coli; PROTEINS; BACTERIA; GENE expression
    • Abstract:
      Reports on the bacterium, Burkholderia sp. strain PS12 which uses 1,2,4,5-tetrachlorobenzene (Cl[sub 4]-benzene) as a growth substrate. Cloning of a 5503-bp DNA fragment from PS12 and expression in Escherichia coli; Inclusion of the tec genes coding for the alpha-subunit and beta-subunit of a terminal dioxygenase, a ferrodoxin and a reductase; Proof that dioxygenase caused dechlorination of Cl[sub 4]-benzene.