Persistent transmission of mouse hepatitis virus by transgenic mice.

Publisher: American Association for Laboratory Animal Science Country of Publication: United States NLM ID: 100900466 Publication Model: Print Cited Medium: Print ISSN: 1532-0820 (Print) Linking ISSN: 15320820 NLM ISO Abbreviation: Comp Med Subsets: MEDLINE

Original Publication: Memphis, TN : American Association for Laboratory Animal Science, 2000-

Coronavirus Infections/*veterinary ; Disease Outbreaks/*veterinary ; Disease Transmission, Infectious/*veterinary ; Hepatitis, Viral, Animal/*transmission ; Mice, Transgenic/*virology ; Receptors, Antigen, T-Cell, alpha-beta/*genetics ; Rodent Diseases/*transmission; Animal Husbandry/methods ; Animals ; Animals, Congenic ; Antibodies, Viral/blood ; Coronavirus Infections/immunology ; Coronavirus Infections/transmission ; Equipment Contamination ; Female ; Hepatitis, Viral, Animal/immunology ; Hepatitis, Viral, Animal/virology ; Housing, Animal ; Immunocompetence ; Infection Control/methods ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Mice, Inbred CBA ; Reverse Transcriptase Polymerase Chain Reaction ; Rodent Diseases/immunology ; Rodent Diseases/virology

Variation in susceptibility to viral infection is well documented across mouse strains. Specific combinations of viral strains and murine hosts may favor viral infection and disease, and could potentially allow the unexpected development of chronic, persistent, or latent infections. In some genetically modified strains of mice, immune function and perhaps other physiologic or metabolic systems may be substantially or marginally impaired. In the case study reported here, we document the apparent persistent transmission of mouse hepatitis virus (MHV) over a two-year period by MHV-seropositive transgenic mice. Transmission occurred via direct contact with seropositive mice and exposure to contaminated bedding. However, MHV was not detected at diagnostic laboratories by use of viral isolation or reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of tissues from MHV-seropositive animals. Our observation, together with the constantly expanding varieties of immune-impaired or poorly characterized murine hosts and the burgeoning dissemination of these animals throughout the biomedical research community, indicate that unexpected pathophysiologic presentations of common murine viral diseases may present new challenges to the biomedical research community in the future.

CA-21765 United States CA NCI NIH HHS

0 (Antibodies, Viral)
0 (Receptors, Antigen, T-Cell, alpha-beta)

Date Created: 20020402 Date Completed: 20040813 Latest Revision: 20081121

20221213

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