Menu
×
John L. Dart Library
9 a.m. – 7 p.m.
Phone: (843) 722-7550
West Ashley Library
9 a.m. – 7 p.m.
Phone: (843) 766-6635
Folly Beach Library
Closed
Phone: (843) 588-2001
Edgar Allan Poe/Sullivan's Island Library
Closed for renovations
Phone: (843) 883-3914
Wando Mount Pleasant Library
9 a.m. – 8 p.m.
Phone: (843) 805-6888
Village Library
9 a.m. – 6 p.m.
Phone: (843) 884-9741
St. Paul's/Hollywood Library
9 a.m. – 8 p.m.
Phone: (843) 889-3300
Otranto Road Library
9 a.m. – 8 p.m.
Phone: (843) 572-4094
Mt. Pleasant Library
9 a.m. – 8 p.m.
Phone: (843) 849-6161
McClellanville Library
9 a.m. - 6 p.m.
Phone: (843) 887-3699
Keith Summey North Charleston Library
9 a.m. – 8 p.m.
Phone: (843) 744-2489
John's Island Library
9 a.m. – 8 p.m.
Phone: (843) 559-1945
Hurd/St. Andrews Library
9 a.m. – 8 p.m.
Phone: (843) 766-2546
Miss Jane's Building (Edisto Library Temporary Location)
9 a.m. – 6 p.m.
Phone: (843) 869-2355
Dorchester Road Library
9 a.m. – 8 p.m.
Phone: (843) 552-6466
Baxter-Patrick James Island
9 a.m. – 8 p.m.
Phone: (843) 795-6679
Main Library
9 a.m. – 8 p.m.
Phone: (843) 805-6930
Bees Ferry West Ashley Library
9 a.m. – 8 p.m.
Phone: (843) 805-6892
Mobile Library
9 a.m. - 5 p.m.
Phone: (843) 805-6909
Today's Hours
John L. Dart Library
9 a.m. – 7 p.m.
Phone: (843) 722-7550
West Ashley Library
9 a.m. – 7 p.m.
Phone: (843) 766-6635
Folly Beach Library
Closed
Phone: (843) 588-2001
Edgar Allan Poe/Sullivan's Island Library
Closed for renovations
Phone: (843) 883-3914
Wando Mount Pleasant Library
9 a.m. – 8 p.m.
Phone: (843) 805-6888
Village Library
9 a.m. – 6 p.m.
Phone: (843) 884-9741
St. Paul's/Hollywood Library
9 a.m. – 8 p.m.
Phone: (843) 889-3300
Otranto Road Library
9 a.m. – 8 p.m.
Phone: (843) 572-4094
Mt. Pleasant Library
9 a.m. – 8 p.m.
Phone: (843) 849-6161
McClellanville Library
9 a.m. - 6 p.m.
Phone: (843) 887-3699
Keith Summey North Charleston Library
9 a.m. – 8 p.m.
Phone: (843) 744-2489
John's Island Library
9 a.m. – 8 p.m.
Phone: (843) 559-1945
Hurd/St. Andrews Library
9 a.m. – 8 p.m.
Phone: (843) 766-2546
Miss Jane's Building (Edisto Library Temporary Location)
9 a.m. – 6 p.m.
Phone: (843) 869-2355
Dorchester Road Library
9 a.m. – 8 p.m.
Phone: (843) 552-6466
Baxter-Patrick James Island
9 a.m. – 8 p.m.
Phone: (843) 795-6679
Main Library
9 a.m. – 8 p.m.
Phone: (843) 805-6930
Bees Ferry West Ashley Library
9 a.m. – 8 p.m.
Phone: (843) 805-6892
Mobile Library
9 a.m. - 5 p.m.
Phone: (843) 805-6909
Patron Login
menu
Item request has been placed!
×
Item request cannot be made.
×
![loading](/sites/all/modules/hf_eds/images/loading.gif)
P3, a monoclonal antibody capable to activate B-1a cells.
Item request has been placed!
×
Item request cannot be made.
×
![loading](/sites/all/modules/hf_eds/images/loading.gif)
- Author(s): Martínez, Darel1 (AUTHOR); Cabrera, Lianet1 (AUTHOR); Hernández, Ana María1 (AUTHOR)
- Source:
Biotecnologia Aplicada. abr-jun2016, Vol. 33 Issue 2, p2211-2216. 6p. 4 Graphs. - Source:
- Additional Information
- Alternate Title: P3, un anticuerpo monoclonal capaz de activar células B-1a.
- Subject Terms:
- Abstract: El P3 es un anticuerpo monoclonal murino de isotipo IgM, que reconoce a los gangliósidos N-glicolilados (un tipo de glicolípidos presente en la membrana celular de la mayoría de los vertebrados), a otros auto-antígenos como los sulfátidos y posee una alta homología con un anticuerpo que reconoce a la mielina de los oligodendrocitos. A pesar de ser una proteína autóloga, previamente se demostró que es capaz de activar una cascada idiotípica de células B y T CD4+ y CD8+ anti-idiotípicas en ausencia de adyuvantes o proteínas transportadoras. Las células B-1a son la población dominante durante las fases tempranas del desarrollo, cuando se establece la conectividad idiotípica, y los anticuerpos naturales de línea germinal secretados por estas células desempeñan un papel fundamental en la protección contra infecciones y el mantenimiento de la homeostasis. En este trabajo se demostró que el AcM P3 es capaz de reconocer a células B-1a de ratones BALB/c vírgenes. Este anticuerpo indujo en dichas células la expresión a altos niveles de los marcadores de activación CD25, CD69 y CD86, la secreción de IgM y un patrón mixto de las citocinas IFN-γ, IL-4 e IL-10. La capacidad del AcM P3 para activar células B-1a puede contribuir a su inmunogenicidad en el modelo singénico y, además, ser usada como una herramienta para direccionar específicamente contra las células B-1a y estimularlas. [ABSTRACT FROM AUTHOR]
- Abstract: P3 is a murine IgM mAb that recognizes N-glycolylated gangliosides, glycolipids expressed on the cell membranes of most vertebrates. It also binds other self-antigens like sulfatides and has high homology with an antibody that recognizes myelin oligodendrocytes. Previously it was demonstrated that P3 mAb is able to activate an idiotypic cascade involving autologous anti-idiotypic B and T cells, in the absence of adjuvant or carrier protein, despite being a self-protein. The anti-P3 idiotype response is mediated not only by CD4+, but also by CD8+ T cells. B-1a cells are the dominant B cell population during the early phases of development, when the idiotypic connectivity is established. Germline natural antibodies secreted by these cells play fundamental roles fighting infections and keeping the homeostasis. In this work, we show that the P3 mAb was able to recognize B-1a cells from naïve BALB/c mice. This antibody induced the overexpression of the activation markers CD25, CD69 and CD86, and increased the secretion of IgM and a mixed pattern of IFN-γ, IL-4 and IL-10 cytokines by B-1a cells. The P3 mAb ability of activating the B-1a cells could contribute to its immunogenicity in the syngeneic model, further supporting its use as a tool to specifically target and stimulate B-1a cells. [ABSTRACT FROM AUTHOR]
- Abstract: Copyright of Biotecnologia Aplicada is the property of Elfos Scientiae and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Alternate Title:
Contact CCPL
Copyright 2022 Charleston County Public Library Powered By EBSCO Stacks 3.3.0 [350.3] | Staff Login
No Comments.