Interaction of an uuter membrane protein of enterotoxigenic Escherichia coli with cell surface heparan sulfate proteoglycans.

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  • Author(s): Fleckenstein JM;Fleckenstein JM; Holland JT; Hasty DL
  • Source:
    Infection and immunity [Infect Immun] 2002 Mar; Vol. 70 (3), pp. 1530-7.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 0246127 Publication Model: Print Cited Medium: Print ISSN: 0019-9567 (Print) Linking ISSN: 00199567 NLM ISO Abbreviation: Infect Immun Subsets: MEDLINE
    • Publication Information:
      Publication: Washington, DC : American Society For Microbiology
      Original Publication: [Bethesda, Md.] American Society for Microbiology.
    • Subject Terms:
      Bacterial Outer Membrane Proteins/*metabolism ; Escherichia coli/*pathogenicity ; Escherichia coli Proteins/*metabolism ; Heparan Sulfate Proteoglycans/*metabolism ; Membrane Proteins/*metabolism; Animals ; Antimicrobial Cationic Peptides ; Bacterial Adhesion ; Blood Proteins/metabolism ; Carrier Proteins/metabolism ; Cell Line ; Cricetinae ; Epithelial Cells/microbiology ; Protein Binding
    • Abstract:
      We have previously shown that enterotoxigenic invasion protein A (Tia), a 25-kDa outer membrane protein encoded on an apparent pathogenicity island of enterotoxigenic Escherichia coli (ETEC) strain H10407, mediates attachment to and invasion into cultured human gastrointestinal epithelial cells. The epithelial cell receptor(s) for Tia has not been identified. Here we show that Tia interacts with cell surface heparan sulfate proteoglycans. Recombinant E. coli expressing Tia mediated invasion into wild-type epithelial cell lines but not invasion into proteoglycan-deficient cells. Furthermore, wild-type eukaryotic cells, but not proteoglycan-deficient eukaryotic cells, attached to immobilized polyhistidine-tagged recombinant Tia (rTia). Binding of epithelial cells to immobilized rTia was inhibited by exogenous heparan sulfate glycosaminoglycans but not by hyaluronic acid, dermatan sulfate, or chondroitin sulfate. Similarly, pretreatment of eukaryotic cells with heparinase I, but not pretreatment of eukaryotic cells with chrondroitinase ABC, inhibited attachment to rTia. In addition, we also observed heparin binding to both immobilized rTia and recombinant E. coli expressing Tia. Heparin binding was inhibited by a synthetic peptide representing a surface loop of Tia, as well as by antibodies directed against this peptide. Additional studies indicated that Tia, as a prokaryotic heparin binding protein, may also interact via sulfated proteoglycan molecular bridges with a number of mammalian heparan sulfate binding proteins. These findings suggest that the binding of Tia to host epithelial cells is mediated at least in part through heparan sulfate proteoglycans and that ETEC belongs on the growing list of pathogens that utilize these ubiquitous cell surface molecules as receptors.
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    • Accession Number:
      0 (AZU1 protein, human)
      0 (Antimicrobial Cationic Peptides)
      0 (Bacterial Outer Membrane Proteins)
      0 (Blood Proteins)
      0 (Carrier Proteins)
      0 (Escherichia coli Proteins)
      0 (Heparan Sulfate Proteoglycans)
      0 (Membrane Proteins)
      0 (enterotoxigenic invasive protein A, E coli)
    • Publication Date:
      Date Created: 20020221 Date Completed: 20020401 Latest Revision: 20210526
    • Publication Date:
      20221213
    • Accession Number:
      PMC127767
    • Accession Number:
      10.1128/IAI.70.3.1530-1537.2002
    • Accession Number:
      11854241