Acceleration of granulocyte colony-stimulating factor-induced neutrophilic nuclear lobulation by overexpression of Lyn tyrosine kinase.

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  • Author(s): Omura T;Omura T; Sakai H; Murakami H
  • Source:
    European journal of biochemistry [Eur J Biochem] 2002 Jan; Vol. 269 (1), pp. 381-9.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies Country of Publication: England NLM ID: 0107600 Publication Model: Print Cited Medium: Print ISSN: 0014-2956 (Print) Linking ISSN: 00142956 NLM ISO Abbreviation: Eur J Biochem Subsets: MEDLINE
    • Publication Information:
      Publication: -2004: Oxford, UK : Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
      Original Publication: Berlin, New York, Springer.
    • Subject Terms:
      Granulocyte Colony-Stimulating Factor/*pharmacology ; Neutrophils/*drug effects ; src-Family Kinases/*physiology; Animals ; Cell Differentiation/drug effects ; Cell Division/drug effects ; Cell Line ; DNA, Complementary/analysis ; Mice ; Neutrophils/physiology ; Peroxidase/genetics ; Phosphorylation ; Tyrosine/metabolism ; src-Family Kinases/genetics
    • Abstract:
      Stimulation with granulocyte colony-stimulating factor (G-CSF) induces myeloid precursor cells to differentiate into neutrophils, and tyrosine phosphorylation of certain cellular proteins is crucial to this process. However, the signaling pathways for neutrophil differentiation are still obscure. As the Src-like tyrosine kinase, Lyn, has been reported to play a role in G-CSF-induced proliferation in avian lymphoid cells, we examined its involvement in G-CSF-induced signal transduction in mammalian cells. Expression plasmids for wild-type Lyn (Lyn) and kinase-negative Lyn (LynKN) were introduced into a murine granulocyte precursor cell line, GM-I62M, that can respond to G-CSF with neutrophil differentiation, and cell lines that overexpressed these molecules (GM-Lyn, GM-LynKN) were established. Upon G-CSF stimulation, both the GM-Lyn and GM-LynKN cells began to differentiate into neutrophils, showing early morphological changes within a few days, much more rapidly than did the parental cells, which started to exhibit nuclear lobulation about 10 days after the cells were transferred to G-CSF-containing medium. However, the time course of expression of the myeloperoxidase gene, another neutrophil differentiation marker, was not affected by the overexpression of Lyn or LynKN. Therefore, in normal cells, protein interactions with Lyn, but not its kinase activity, are important for the induction of G-CSF-induced neutrophilic nuclear lobulation in mammalian granulopoiesis.
    • Accession Number:
      0 (DNA, Complementary)
      143011-72-7 (Granulocyte Colony-Stimulating Factor)
      42HK56048U (Tyrosine)
      EC 1.11.1.7 (Peroxidase)
      EC 2.7.10.2 (lyn protein-tyrosine kinase)
      EC 2.7.10.2 (src-Family Kinases)
    • Publication Date:
      Date Created: 20020111 Date Completed: 20020221 Latest Revision: 20190620
    • Publication Date:
      20221213
    • Accession Number:
      10.1046/j.0014-2956.2001.02661.x
    • Accession Number:
      11784333