[The effects of lipopolysaccharide and anti-inflammatory drugs on nuclear factor-kappa B in pulmonary intravascular macrophage].

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Author(s): Cai E;Cai E; Chen Z; Wu W
  • Source:
    Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases [Zhonghua Jie He He Hu Xi Za Zhi] 1999 May; Vol. 22 (5), pp. 283-6.
  • Publication Type:
    English Abstract; Journal Article
  • Language:
    Chinese
  • Additional Information
    • Source:
      Publisher: Chinese Medical Association Country of Publication: China NLM ID: 8712226 Publication Model: Print Cited Medium: Print ISSN: 1001-0939 (Print) Linking ISSN: 10010939 NLM ISO Abbreviation: Zhonghua Jie He He Hu Xi Za Zhi Subsets: MEDLINE
    • Publication Information:
      Publication: Beijing : Chinese Medical Association
      Original Publication: Beijing : Zhonghua yi xue hui, 1987-
    • Subject Terms:
      Anti-Inflammatory Agents/*pharmacology ; Anti-Inflammatory Agents, Non-Steroidal/*pharmacology ; Aspirin/*pharmacology ; Dexamethasone/*pharmacology ; Glucocorticoids/*pharmacology ; Lipopolysaccharides/*pharmacology ; Macrophages, Alveolar/*drug effects ; Mitogens/*pharmacology ; NF-kappa B/*metabolism; Animals ; Cells, Cultured ; Culture Media ; Electrophoretic Mobility Shift Assay ; Humans ; Macrophages, Alveolar/cytology ; Macrophages, Alveolar/metabolism ; Sensitivity and Specificity ; Swine ; Tumor Necrosis Factor-alpha/biosynthesis
    • Abstract:
      Objective: To investigate activation of nuclear factor-kappa B(NF-kappa B) in pulmonary intravascular macrophage (PIM) stimulated with lipopolysaccharide (LPS) and effects of anti-inflammatory drugs dexamethasone (DEX) and aspirin (ASA) on the process.
      Methods: PIMs isolated from three healthy pigs were cultured and divided into four groups: Control group; LPS- stimulated group; DEX- or ASA-treated group. The NF-kappa B activity of nuclear protein extract from the PIMs and the concentration of tumor necrosis factor alpha (TNF-alpha) in the supernatant were measured by electrophoretic mobility shift assay (EMSA) and radioimmunoassay (RIA), respectively.
      Results: The activity of NF-kappa B significantly increased from 0.5 hr to 4 hr after LPS stimulation (P < 0.01); The level of TNF-alpha in the supernatant elevated markedly from 1 hr to 2 hr after LPS stimulation (P < 0.01); positive correlation was found between the NF-kappa B activity and the TNF-alpha concentration at 1 hr after LPS stimulation (r = 0.991, P < 0.01). Compared with LPS-stimulated group, both NF-kappa B activity and TNF-alpha concentration were significantly lowered in DEX- or ASA-treated groups (P < 0.01).
      Conclusions: LPS might activate NF-kappa B in the PIMs, and induce the increase of transcription and expression of TNF-alpha gene; Both DEX and ASA could inhibit the activation of NF-kappa B and reduce the release of TNF-alpha.
    • Accession Number:
      0 (Anti-Inflammatory Agents)
      0 (Anti-Inflammatory Agents, Non-Steroidal)
      0 (Culture Media)
      0 (Glucocorticoids)
      0 (Lipopolysaccharides)
      0 (Mitogens)
      0 (NF-kappa B)
      0 (Tumor Necrosis Factor-alpha)
      7S5I7G3JQL (Dexamethasone)
      R16CO5Y76E (Aspirin)
    • Publication Date:
      Date Created: 20020105 Date Completed: 20020131 Latest Revision: 20131121
    • Publication Date:
      20231215
    • Accession Number:
      11775855