Improvement in the bioconversion of penicillin G to deacetoxycephalosporin G by elimination of agitation and addition of decane.

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  • Author(s): Gao Q;Gao Q; Demain AL
  • Source:
    Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2001 Nov; Vol. 57 (4), pp. 511-3.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Print Cited Medium: Print ISSN: 0175-7598 (Print) Linking ISSN: 01757598 NLM ISO Abbreviation: Appl Microbiol Biotechnol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Berlin ; New York : Springer International, c1984-
    • Subject Terms:
      Alkanes/*pharmacology ; Cephalosporins/*metabolism ; Penicillin G/*metabolism ; Streptomyces/*metabolism; Biotransformation
    • Abstract:
      The bioconversion of penicillin G to deacetoxycephalosporin G (DAOG) using resting cells of Streptomyces clavuligerus could be a very valuable step in the economical production of semisynthetic cephalosporin antibiotics. The extent of the reaction, however, is very low due to inactivation of the ring expansion enzyme deacetoxycephalosporin C synthetase ("expandase") by reaction components. We show that elimination of agitation during the reaction lowers the rate but increases the amount of DAOG produced, presumably because the inactivation requires high levels of oxygen. Many additives to the medium were examined for their effect on the reaction. Clearly, the most effective compound was the organic solvent, decane.
    • Contributed Indexing:
      Keywords: Non-programmatic
    • Accession Number:
      0 (Alkanes)
      0 (Cephalosporins)
      0 (deacetoxycephalosporin G)
      NK85062OIY (decane)
      Q42T66VG0C (Penicillin G)
    • Publication Date:
      Date Created: 20020105 Date Completed: 20020516 Latest Revision: 20190916
    • Publication Date:
      20221213
    • Accession Number:
      10.1007/s002530100817
    • Accession Number:
      11762597