Enhanced apoptosis under low serum conditions in human glioblastoma cells by connexin 43 (Cx43).

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  • Author(s): Huang R;Huang R; Liu YG; Lin Y; Fan Y; Boynton A; Yang D; Huang RP
  • Source:
    Molecular carcinogenesis [Mol Carcinog] 2001 Nov; Vol. 32 (3), pp. 128-38.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Wiley-Liss Country of Publication: United States NLM ID: 8811105 Publication Model: Print Cited Medium: Print ISSN: 0899-1987 (Print) Linking ISSN: 08991987 NLM ISO Abbreviation: Mol Carcinog Subsets: MEDLINE
    • Publication Information:
      Publication: <2005- > : [Hoboken, N.J.] : Wiley-Liss
      Original Publication: New York : Alan R. Liss, Inc., c1988-
    • Subject Terms:
      Apoptosis/*genetics ; Connexin 43/*genetics ; Glioblastoma/*genetics ; Glioblastoma/*pathology; Chromones/pharmacology ; Culture Media ; Estrogens, Non-Steroidal/pharmacology ; Gene Expression Regulation, Neoplastic ; Genes, bcl-2 ; Humans ; Morpholines/pharmacology ; Phosphoinositide-3 Kinase Inhibitors ; Tumor Cells, Cultured
    • Abstract:
      Connexin 43 (Cx43), a structural component of gap junctions, is believed to function as a tumor suppressor gene. Previously, we showed that expression of Cx43 suppresses cell proliferation and tumorigenicity of human glioblastoma cells [Huang et al., Cancer Res 58:5089-5096, 1998] and enhances apoptosis in response to chemotherapeutic agents [Huang et al., Int J Cancer 92:130-138, 2001]. In the present study, we demonstrated that expression of Cx43 in human glioblastoma cells potentiated an apoptotic program under low-serum conditions. The Cx43-mediated effect was coupled with a decreased expression of the specific apoptosis-inhibitor bcl-2. Overexpression of bcl-2 in Cx43-transfected cells conferred resistance to apoptosis induced under low-serum conditions, suggesting that the Cx43-mediated apoptosis under low-serum conditions is regulated, in part, through the downregulation of bcl-2 expression. Furthermore, application of the phosphatidylinositol-3'-OH kinase inhibitor LY294002 specifically induced apoptosis in Cx43-transfected cells. Our results demonstrate a new role of Cx43 in the mediation of apoptosis under low serum conditions.
      (Copyright 2001 Wiley-Liss, Inc.)
    • Grant Information:
      CA 39745 United States CA NCI NIH HHS; CA 89273 United States CA NCI NIH HHS
    • Accession Number:
      0 (Chromones)
      0 (Connexin 43)
      0 (Culture Media)
      0 (Estrogens, Non-Steroidal)
      0 (Morpholines)
      0 (Phosphoinositide-3 Kinase Inhibitors)
      31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one)
    • Publication Date:
      Date Created: 20011218 Date Completed: 20011221 Latest Revision: 20191210
    • Publication Date:
      20240829
    • Accession Number:
      10.1002/mc.1072
    • Accession Number:
      11746825