Evaluation of a near-patient test and 2 enzyme-linked immunosorbent assay-based assays for detecting anti-herpes simplex virus type-2 antibodies.

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  • Author(s): Palù G;Palù G; Calistri A; Cancellotti E; Cusan M; Mengoli C
  • Source:
    Scandinavian journal of infectious diseases [Scand J Infect Dis] 2001; Vol. 33 (10), pp. 794-6.
  • Publication Type:
    Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Corporate Authors:
      Italian Herpes Forum
    • Source:
      Publisher: Informa Healthcare Country of Publication: England NLM ID: 0215333 Publication Model: Print Cited Medium: Print ISSN: 0036-5548 (Print) Linking ISSN: 00365548 NLM ISO Abbreviation: Scand J Infect Dis Subsets: MEDLINE
    • Publication Information:
      Publication: 2007-2014 : London : Informa Healthcare
      Original Publication: Stockholm, Society for the Publication of Acta medica Scandinavica.
    • Subject Terms:
      Reagent Kits, Diagnostic*; Antibodies, Viral/*analysis ; Enzyme-Linked Immunosorbent Assay/*methods ; Herpesvirus 1, Human/*immunology ; Herpesvirus 2, Human/*immunology; Blotting, Western/methods ; Double-Blind Method ; Humans ; Sensitivity and Specificity
    • Abstract:
      Several type-specific serologic assays for herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2), based on glycoprotein G1 (gG1) and gG2, have recently been developed. These include immunodot (POCkit HSV-2) and enzyme-linked immunosorbent assay (ELISA). The diagnostic value of POCkit HSV-2, a near-patient test, and of 2 immunoenzymatic, type-specific assays was evaluated on 122 patients attending an STD clinic. Western blot was used as the reference test. The sensitivity of POCkit HSV-2 was good but the specificity was poor, so that in a population with low seroprevalence, a positive result is likely to be a false positive. Analysis of 2 currently available HSV type-specific ELISAs yielded results suggesting that the sensitivity of these tests may also be suboptimal.
    • Accession Number:
      0 (Antibodies, Viral)
      0 (Reagent Kits, Diagnostic)
    • Publication Date:
      Date Created: 20011201 Date Completed: 20020221 Latest Revision: 20191210
    • Publication Date:
      20240829
    • Accession Number:
      10.1080/003655401317074716
    • Accession Number:
      11728057