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Co-translational folding of caspase-activated DNase with Hsp70, Hsp40, and inhibitor of caspase-activated DNase.
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- Author(s): Sakahira H;Sakahira H; Nagata S
- Source:
The Journal of biological chemistry [J Biol Chem] 2002 Feb 01; Vol. 277 (5), pp. 3364-70. Date of Electronic Publication: 2001 Nov 27.
- Publication Type:
Journal Article; Research Support, Non-U.S. Gov't
- Language:
English
- Additional Information
- Source:
Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Print ISSN: 0021-9258 (Print) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE
- Publication Information:
Publication: 2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology
Original Publication: Baltimore, MD : American Society for Biochemistry and Molecular Biology
- Subject Terms:
- Abstract:
CAD (caspase-activated DNase) that causes chromosomal DNA fragmentation during apoptosis exists as a complex with ICAD (inhibitor of CAD) in proliferating cells. Here, we report that denatured CAD is functionally refolded with Hsc70-Hsp40 and ICAD. Hsc70-Hsp40 suppresses the aggregation of the denatured CAD, but cannot restore its enzymatic activity. In contrast, ICAD could not suppress the aggregation of CAD, but supported the CAD's renaturation with Hsc70-Hsp40, indicating that ICAD recognizes the quasi-native folding state of CAD that is conferred by Hsc70-Hsp40. Using an in vitro translation system, we then showed that during CAD translation, Hsc70-Hsp40 as well as ICAD bind to the nascent CAD polypeptide, while on ribosomes. These results indicate that ICAD together with Hsc70-Hsp40 assists the folding of CAD during its synthesis, and that the CAD*ICAD heterodimer is formed co-translationally.
- Accession Number:
0 (Apoptosis Regulatory Proteins)
0 (Epitopes)
0 (HSP40 Heat-Shock Proteins)
0 (HSP70 Heat-Shock Proteins)
0 (Heat-Shock Proteins)
0 (Peptide Fragments)
0 (Proteins)
0 (Recombinant Proteins)
0 (caspase-activated DNase inhibitor)
8L70Q75FXE (Adenosine Triphosphate)
EC 3.1.- (Deoxyribonucleases)
EC 3.1.- (caspase-activated deoxyribonuclease)
- Publication Date:
Date Created: 20011129 Date Completed: 20020228 Latest Revision: 20210209
- Publication Date:
20240829
- Accession Number:
10.1074/jbc.M110071200
- Accession Number:
11724800
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