[Involvement of regulating the phosphatidylinositol 3-kinase signaling pathway in the process of herbimycin A-induced erythroid cell differentiation in the K562 erythroleukemia cell line].

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  • Additional Information
    • Transliterated Title:
      Vyvchennia rehuliatsiï fosfatydylinozytol 3-kinaznoho syhnal'noho shliakhu v protsesi herbimitsyn A-indukovanoï erytroïdnoï dyferentsiatsiï klityn erytroleikemiï liniï K562.
    • Source:
      Publisher: Nat︠s︡ionalʹna akademii︠a︡ nauk Ukraïny, Instytut biokhimiï im. O.V. Palladina Country of Publication: Ukraine NLM ID: 101657175 Publication Model: Print Cited Medium: Print NLM ISO Abbreviation: Ukr Biokhim Zh (1999) Subsets: MEDLINE
    • Publication Information:
      Original Publication: Kyïv : Nat︠s︡ionalʹna akademii︠a︡ nauk Ukraïny, Instytut biokhimiï im. O.V. Palladina, 1999-2013.
    • Subject Terms:
      Signal Transduction*; Anti-Bacterial Agents/*pharmacology ; Cell Differentiation/*drug effects ; Erythrocytes/*drug effects ; Phosphatidylinositol 3-Kinases/*metabolism ; Quinones/*pharmacology; Benzoquinones ; Erythrocytes/cytology ; Glutathione Transferase/metabolism ; Humans ; K562 Cells ; Lactams, Macrocyclic ; Protein Binding ; Recombinant Fusion Proteins/metabolism ; Rifabutin/analogs & derivatives ; src Homology Domains
    • Abstract:
      The changes in protein-protein interactions mediated by SH3 domain of the regulatory p85 alpha subunit of phosphatidylinositol 3-kinase (Pl 3-kinase) in the course of herbimycin A-induced erythroid differentiation of the human erythroleukemia cell line K562 have been analyzed. Binding assay was performed in vitro using a recombinant form of SH3 domain of p85 alpha, conjugated with glutathione-S-transferase. pTyr-containing 210, 116, 52 and 46 kDa proteins, binding of which are modulated in differentiated cells, were identified and binding dynamics was analysed. The obtained data on binding the specific pTyr-containing proteins with regulatory subunit of Pl 3-kinase testify about the coordinated control of Pl 3-kinase signalling pathway in the course of herbimycin A-induced K562 cells differentiation.
    • Accession Number:
      0 (Anti-Bacterial Agents)
      0 (Benzoquinones)
      0 (Lactams, Macrocyclic)
      0 (Quinones)
      0 (Recombinant Fusion Proteins)
      1W306TDA6S (Rifabutin)
      70563-58-5 (herbimycin)
      EC 2.5.1.18 (Glutathione Transferase)
      EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
    • Publication Date:
      Date Created: 20011020 Date Completed: 20020116 Latest Revision: 20150803
    • Publication Date:
      20240829
    • Accession Number:
      11642032