Use of detergent extracts of Brucella abortus RB51 to detect serologic responses in RB51-vaccinated cattle.

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  • Author(s): Halling SM;Halling SM; Koster NA
  • Source:
    Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc [J Vet Diagn Invest] 2001 Sep; Vol. 13 (5), pp. 408-12.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Sage Country of Publication: United States NLM ID: 9011490 Publication Model: Print Cited Medium: Print ISSN: 1040-6387 (Print) Linking ISSN: 10406387 NLM ISO Abbreviation: J Vet Diagn Invest Subsets: MEDLINE
    • Publication Information:
      Publication: 2011-: Thousand Oaks, CA : Sage
      Original Publication: 1989-2004 : Columbia, MO : American Association of Veterinary Laboratory Diagnosticians
    • Subject Terms:
      Antigens, Bacterial/*analysis ; Brucella abortus/*immunology ; Brucellosis, Bovine/*diagnosis ; Detergents/*chemistry ; Octoxynol/*chemistry ; Quaternary Ammonium Compounds/*chemistry ; Sodium Dodecyl Sulfate/*chemistry ; Vaccination/*veterinary; Animals ; Bacterial Vaccines ; Blotting, Western ; Brucella abortus/isolation & purification ; Brucella abortus/pathogenicity ; Brucellosis, Bovine/immunology ; Cattle ; Molecular Weight ; Serologic Tests/veterinary ; Specimen Handling
    • Abstract:
      Serologic responses to the newly introduced rough Brucella abortus vaccine strain RB51 have been determined in a dot-blot format using gamma-irradiated RB51 cells as the antigen. Because gamma-irradiated cells are not easily prepared and the signal from cells was not always reliable, an alternative antigen was sought. Detergent extracts of B. abortus RB51 were prepared using zwittergent 3-14, Triton X-100, and sodium dodecyl sulfate (SDS) and examined in a dot-blot format. Zwittergent 3-14 extracts and gamma-irradiated RB51 cells gave the same titers. Unlike gamma-irradiated RB51 cells, zwittergent 3-14 extracts produced signals consistently, and the signals were easily interpreted. Triton X-100 extracts interfered with signal development, and SDS extracts resulted in a high background signal. Western blot analyses revealed several outer membrane proteins in the zwittergent 3-14 extract. The major antigens in the extract had apparent molecular weights of <20,000.
    • Accession Number:
      0 (Antigens, Bacterial)
      0 (Bacterial Vaccines)
      0 (Detergents)
      0 (Quaternary Ammonium Compounds)
      14933-09-6 (zwittergent 3-14)
      368GB5141J (Sodium Dodecyl Sulfate)
      9002-93-1 (Octoxynol)
    • Publication Date:
      Date Created: 20011003 Date Completed: 20020129 Latest Revision: 20190522
    • Publication Date:
      20231215
    • Accession Number:
      10.1177/104063870101300507
    • Accession Number:
      11580062