Effects of high doses of vitamin D on mucosa-associated gut microbiome vary between regions of the human gastrointestinal tract.

MICROBIOLOGY; FECES; GUT microbiome; T cells; BIOPSY; CLINICAL trials; COLON (Anatomy); DIETARY supplements; FLOW cytometry; INTESTINAL mucosa; SMALL intestine; MULTIVARIATE analysis; ORAL drug administration; POLYMERASE chain reaction; PROBABILITY theory; RESEARCH funding; STATISTICS; STOMACH; T-test (Statistics); PILOT projects; DATA analysis; CHOLECALCIFEROL; BODY mass index; PRE-tests & post-tests; DESCRIPTIVE statistics; ENDOSCOPIC gastrointestinal surgery; SEQUENCE analysis; PHYSIOLOGY

Purpose: Vitamin D is well known for its effects on bone mineralisation but has also been attributed immunomodulatory properties. It positively influences human health, but in vivo data describing vitamin D effects on the human gut microbiome are missing. We aimed to investigate the effects of oral vitamin D supplementation on the human mucosa-associated and stool microbiome as well as CD8 T cells in healthy volunteers. Methods: This was an interventional, open-label, pilot study. Sixteen healthy volunteers (7 females, 9 males) were endoscopically examined to access a total of 7 sites. We sampled stomach, small bowel, colon, and stools before and after 8 weeks of vitamin D supplementation. Bacterial composition was assessed by pyrosequencing the 16S rRNA gene (V1-2), and CD8 T cell counts were determined by flow cytometry. Results: Vitamin D supplementation changed the gut microbiome in the upper GI tract (gastric corpus, antrum, and duodenum). We found a decreased relative abundance of Gammaproteobacteria including Pseudomonas spp. and Escherichia/ Shigella spp. and increased bacterial richness. No major changes occurred in the terminal ileum, appendiceal orifice, ascending colon, and sigmoid colon or in stools, but the CD8 T cell fraction was significantly increased in the terminal ileum. Conclusion: Vitamin D modulates the gut microbiome of the upper GI tract which might explain its positive influence on gastrointestinal diseases, such as inflammatory bowel disease or bacterial infections. The local effects of vitamin D demonstrate pronounced regional differences in the response of the GI microbiome to external factors, which should be considered in future studies investigating the human microbiome. [ABSTRACT FROM AUTHOR]

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