Induction of Mitochondria Mediated Apoptosis in Human Breast Cancer Cells (T-47D) by Annona reticulata L . Leaves Methanolic Extracts.

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    • Subject Terms:
      BREAST tumor diagnosis; PHYTOTHERAPY; ANALYSIS of variance; APOPTOSIS; CELL culture; CELL cycle; CELL physiology; GENE expression; GENES; GROWTH factors; INFLAMMATION; METHANOL; NUTRITION; PROTEINS; RESEARCH funding; T-test (Statistics); PLANT extracts; DATA analysis
    • Subject Terms:
      INDIA
    • Abstract:
      Annona reticulataLinn. (Common name: Bullock's-heart) (Annonaceaefamily) is a semi-evergreen and small deciduous tree. The extracts of various parts ofAnnona reticulataL. have been reported as cytotoxic to many cancer cells.Annona reticulataL. leaves' methanolic extract (ARME) was prepared and used against the breast cancer cells. The breast cancer cells (T-47D) viability and IC50 were evaluated by Vybrant® MTT Cell Proliferation Assay Kit. Detection of phosphatidylserine on membranes of apoptotic cells was done by Attune flow cytometer. RNA transcripts were quantified in ARME treated and untreated cells. Finally, the Vybrant® FAM Poly Caspases assay kit was used for analysis of polycaspases activity in T-47D cells. The IC50 (5 ± 0.5 µg/mL) of the ARME was found against breast cancer cells (T-47D). The Paclitaxel was used as a control standard drug for the study. The downregulation of Bcl-2 and upregulation of Bax and Bak, and caspases activation suggested induction of apoptosis in T-47D cells by ARME through mitochondrial pathway. The cell cycle halted at G2/M phase in the ARME treated cells. The ARME was found to be effective against Breast cancer cells (T-47D). [ABSTRACT FROM PUBLISHER]
    • Abstract:
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