Novel methods for studying lipids and lipases and their mutual interaction at interfaces. Part I. Atomic force microscopy.

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  • Additional Information
    • Source:
      Publisher: Editions Scientifiques Elsevier Country of Publication: France NLM ID: 1264604 Publication Model: Print Cited Medium: Print ISSN: 0300-9084 (Print) Linking ISSN: 03009084 NLM ISO Abbreviation: Biochimie Subsets: MEDLINE
    • Publication Information:
      Publication: Paris : Editions Scientifiques Elsevier
      Original Publication: Paris.
    • Subject Terms:
      Biochemistry/*methods ; Lipase/*chemistry ; Lipids/*chemistry ; Microscopy, Atomic Force/*instrumentation ; Microscopy, Atomic Force/*methods; 1,2-Dipalmitoylphosphatidylcholine/chemistry ; Hydrolysis ; Kinetics ; Lipase/metabolism ; Lipid Bilayers ; Lipid Metabolism ; Models, Biological ; Phospholipases A/chemistry ; Protein Binding ; Time Factors
    • Abstract:
      Mono-layers of lipids and their interaction with surface active enzymes (lipases) have been studied for more than a century. During the past decade new insight into this area has been obtained due to the development of scanning probe microscopy. This novel method provides direct microscopic information about the system in question and allows in situ investigations under near physiological conditions. In the present review the theory, experimental set-up and sample requirements of atomic force microscopy (AFM) are described. An overview of recent results is also presented with special emphasis on lipase hydrolysis and kinetics investigated in situ using AFM.
    • Number of References:
      91
    • Accession Number:
      0 (Lipid Bilayers)
      0 (Lipids)
      2644-64-6 (1,2-Dipalmitoylphosphatidylcholine)
      EC 3.1.1.3 (Lipase)
      EC 3.1.1.32 (Phospholipases A)
    • Publication Date:
      Date Created: 20010523 Date Completed: 20010726 Latest Revision: 20190822
    • Publication Date:
      20231215
    • Accession Number:
      10.1016/s0300-9084(01)01264-0
    • Accession Number:
      11368846