Polyamine cytotoxicity in the presence of bovine serum amine oxidase.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print Cited Medium: Print ISSN: 0006-291X (Print) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE
    • Publication Information:
      Publication: <2002- >: San Diego, CA : Elsevier
      Original Publication: New York, Academic Press.
    • Subject Terms:
      Amine Oxidase (Copper-Containing)/*blood ; Spermidine/*pharmacology ; Spermine/*pharmacology; 3T3 Cells ; Acrolein/metabolism ; Aldehydes/pharmacology ; Animals ; Cattle ; Hydrogen Peroxide/pharmacology ; Mice
    • Abstract:
      The toxicity of extracellular spermine, determined in the presence of fetal calf serum, was studied using three cell lines: FM3A, L1210, and NIH3T3 cells. Amine oxidase in fetal calf serum produces aminodialdehyde generating acrolein spontaneously, H(2)O(2), and ammonia from spermine. Spermine toxicity was prevented by aldehyde dehydrogenase, but not by catalase. Similar concentrations of spermine and acrolein were needed to produce toxicity. Other aldehydes (formaldehyde, acetaldehyde, and propionaldehyde) and hydrogen peroxide were less toxic than acrolein. Spermidine and 3-aminopropanal, which produces acrolein, also exhibited severe cytotoxicity. The degree of cytotoxicity of spermine, spermidine, and 3-aminopropanal was nearly parallel with the amount of acrolein produced from each compound. Thus, it was deduced that acrolein is a major toxic compound produced from polyamines (spermine and spermidine) by amine oxidase.
      (Copyright 2001 Academic Press.)
    • Accession Number:
      0 (Aldehydes)
      2FZ7Y3VOQX (Spermine)
      7864XYD3JJ (Acrolein)
      BBX060AN9V (Hydrogen Peroxide)
      EC 1.4.3.21 (Amine Oxidase (Copper-Containing))
      U87FK77H25 (Spermidine)
    • Publication Date:
      Date Created: 20010327 Date Completed: 20010412 Latest Revision: 20220311
    • Publication Date:
      20231215
    • Accession Number:
      10.1006/bbrc.2001.4569
    • Accession Number:
      11263996