[Serological diagnostic trial of the causative virus of Bell's palsy by anti-herpes virus antibodies in the paired sera].

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  • Author(s): Chida K;Chida K; Takase S
  • Source:
    Rinsho shinkeigaku = Clinical neurology [Rinsho Shinkeigaku] 2000 Aug; Vol. 40 (8), pp. 791-6.
  • Publication Type:
    Clinical Trial; English Abstract; Journal Article
  • Language:
    Japanese
  • Additional Information
    • Source:
      Publisher: Nihon Shinkei Gakkai Country of Publication: Japan NLM ID: 0417466 Publication Model: Print Cited Medium: Print ISSN: 0009-918X (Print) Linking ISSN: 0009918X NLM ISO Abbreviation: Rinsho Shinkeigaku Subsets: MEDLINE
    • Publication Information:
      Publication: April 1963-: Tōkyō : Nihon Shinkei Gakkai
      Original Publication: Tōkyō : Nihon Rinshō Shinkei Gakkai, 1960-
    • Subject Terms:
      Antibodies, Viral/*blood ; Bell Palsy/*virology ; Serologic Tests/*methods; Adult ; Bell Palsy/diagnosis ; Female ; Herpesvirus 3, Human/immunology ; Herpesvirus 3, Human/pathogenicity ; Humans ; Immunoenzyme Techniques/methods ; Immunoglobulin G/blood ; Immunoglobulin M/blood ; Male ; Middle Aged ; Sensitivity and Specificity ; Simplexvirus/immunology ; Simplexvirus/pathogenicity
    • Abstract:
      Substantial evidence obtained through polymerase chain reaction techniques strongly supports that reactivation of latently infected herpes simplex virus (HSV) in geniculate ganglia is the main cause of Bell's palsy. However, serum antibody titers to HSV rarely increase in patients with this disease. This discrepancy may result from the difficulty in detecting a small increase in antibody titers by conventional serological analysis. To detect such a small increase in antibody titer, we defined the significant increase in IgG antibody titers more precisely, and examined the association of HSV or varicella-zoster virus (VZV) with Bell's palsy. From 40 patients with Bell's palsy, paired sera were obtained within the 4th disease day and 2 weeks later. IgG antibodies to HSV, VZV, cytomegalovirus (CMV), or measles virus (MsV) were measured with solid-phase enzyme immunoassay (EIA). IgM antibodies were measured with captured EIA. Each antibody titer was expressed as an EIA-value, which was quantitatively measured by using a calibration curve prepared from the samples containing known titers to the virus in proportion to the logarithm of its titer. An EIA-value ratio (EVR) between paired sera and a corrected EVR was calculated according to the formulae: EVR = [EIA-value in the second serum]/[EIA-value in the first serum]; corrected EVR = [EVR to HSV, VZV or CMV]/[EVR to MsV]. The association with a virus was determined to be positive when the corrected EVR of the virus was beyond the normal range (the logarithmical mean +/- 3 SD of corrected EVRs among 24 healthy controls) while the corrected EVR of the other viruses were within it. Using the corrected EVR, 6 patients were positive: 4 for HSV, 2 for VZV. On the other hand, the conventional serological analysis, which confirms positivity by a 4-fold increase in IgG antibody titer or a demonstration of IgM antibody, disclosed only 2 positive patients (1 for HSV, 1 for VZV). EIA is a very sensitive method of detecting antibodies. Corrected EVRs can exclude a decrease in antibody titers induced by corticosteroids, which are generally used for therapy of Bell's palsy. Moreover, the normal range of corrected EVRs can be defined more precisely than in conventional serological analyses. Our results indicate that some sensitive analysis, such as the corrected EVR method, may make it possible to serologically detect the causative virus of Bell's palsy.
    • Accession Number:
      0 (Antibodies, Viral)
      0 (Immunoglobulin G)
      0 (Immunoglobulin M)
    • Publication Date:
      Date Created: 20010224 Date Completed: 20010419 Latest Revision: 20061115
    • Publication Date:
      20221213
    • Accession Number:
      11218698