Posttransfusion survival (24-hour) and hemolysis of previously frozen, deglycerolized RBCs after storage at 4 degrees C for up to 14 days in sodium chloride alone or sodium chloride supplemented with additive solutions.

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  • Additional Information
    • Source:
      Publisher: American Association Of Blood Banks Country of Publication: United States NLM ID: 0417360 Publication Model: Print Cited Medium: Print ISSN: 0041-1132 (Print) Linking ISSN: 00411132 NLM ISO Abbreviation: Transfusion Subsets: MEDLINE
    • Publication Information:
      Original Publication: Arlington, Va. : American Association Of Blood Banks
    • Subject Terms:
      Blood Preservation* ; Blood Transfusion* ; Cryopreservation* ; Hemolysis*; Adenine/*pharmacology ; Cryoprotective Agents/*metabolism ; Erythrocyte Aging/*physiology ; Erythrocytes/*metabolism ; Glucose/*pharmacology ; Glycerol/*metabolism ; Mannitol/*pharmacology ; Sodium Chloride/*pharmacology; Humans ; Male ; Time Factors
    • Abstract:
      Background: Previously frozen human RBCs currently are glycerolized and deglycerolized by the use of open systems that limit storage of the deglycerolized RBCs at 4 degrees C to only 24 hours.
      Study Design and Methods: Healthy male volunteers who met AABB requirements for blood donors (n = 38) were studied. A volume of 450 mL of blood was collected into CPDA-1. The RBC concentrates were stored at 4 degrees C for 3 to 6 days before being frozen with 40-percent (wt/vol) glycerol and stored at -80 degrees C. The RBCs were deglycerolized, resuspended in 0.9-percent sodium chloride and 0.2-percent glucose (SG) solution or SG solution supplemented with AS-1, AS-3, or AS-5, and stored in the resuspension medium at 4 degrees C for 14 days.
      Results: The mean +/- SD freeze-thaw-wash process recovery was 90.0 +/- 4.0 percent for all 38 units. The mean 24-hour posttransfusion survival value was 79 percent for deglycerolized RBC stored at 4 degrees C for 7 days in SG alone, SG plus AS-3, or SG plus AS-5. Deglycerolized RBC that were stored at 4 C for 14 days in SG supplemented with AS-1, AS-3, or AS-5 had a mean 24-hour posttransfusion survival of 74 percent. After 7 days of storage of deglycerolized RBCs in SG alone, the mean hemolysis was 3. 7 percent. After 14 days of storage of deglycerolized RBCs in SG supplemented with AS-1, AS-3, or AS-5, the mean hemolysis was 2.5 percent.
      Conclusions: The levels of hemolysis did not correlate with the 24-hour posttransfusion survival values.
    • Accession Number:
      0 (ADSOL)
      0 (Cryoprotective Agents)
      3OWL53L36A (Mannitol)
      451W47IQ8X (Sodium Chloride)
      IY9XDZ35W2 (Glucose)
      JAC85A2161 (Adenine)
      PDC6A3C0OX (Glycerol)
    • Publication Date:
      Date Created: 20001202 Date Completed: 20001222 Latest Revision: 20190727
    • Publication Date:
      20250114
    • Accession Number:
      10.1046/j.1537-2995.2000.40111337.x
    • Accession Number:
      11099661