Identification of a common receptor for three types of rat cytokine-induced neutrophil chemoattractants (CINCs).

Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 9005353 Publication Model: Print Cited Medium: Print ISSN: 1043-4666 (Print) Linking ISSN: 10434666 NLM ISO Abbreviation: Cytokine Subsets: MEDLINE

Publication: <2001- > : Oxford : Elsevier Science Ltd.
Original Publication: [Philadelphia, PA] : Saunders Scientific Publications, W.B. Saunders, [c1989-

Chemokines, CXC* ; Intercellular Signaling Peptides and Proteins*; Chemotactic Factors/*genetics ; Chemotactic Factors/*metabolism ; Growth Substances/*genetics ; Growth Substances/*metabolism; Animals ; Blotting, Northern ; Calcium/metabolism ; Cell Line ; Chemokine CXCL1 ; Chemokine CXCL2 ; Chemotactic Factors/pharmacology ; Chemotactic Factors/physiology ; Chemotaxis/drug effects ; Cloning, Molecular ; DNA, Complementary/metabolism ; Dose-Response Relationship, Drug ; Electrophoresis, Polyacrylamide Gel ; Growth Substances/pharmacology ; Growth Substances/physiology ; Humans ; Liver/metabolism ; Mutagenesis ; N-Formylmethionine Leucyl-Phenylalanine/pharmacology ; Neutrophils/metabolism ; Polymerase Chain Reaction ; Rats ; Receptors, Interleukin-8B/genetics ; Receptors, Interleukin-8B/metabolism ; Spectrophotometry ; Time Factors ; Transfection

Rat cytokine-induced neutrophil chemoattractant-1 (CINC-1), CINC-2 and CINC-3/macrophage inflammatory protein-2 (MIP-2), members of the CXC chemokine family, are potent chemotactic factors for neutrophils. In order to identify the receptor for CINCs, rat CXC chemokine receptor 2 (CXCR2) was cloned and expressed in HEK293 cells. CINC-1, CINC-2 and CINC-3 induced calcium mobilizations dose-dependently in CXCR2-transfected cells, whereas formyl-methionyl-leucyl-phenylalanine (FMLP) did not. CINC-3 induced enhancement of cytoplasmic calcium concentration more potently than CINC-1 and CINC-2, and desensitized calcium transients induced by CINC-1 and CINC-2, which were essentially identical to those observed in rat neutrophils. In addition, anti-CXCR2 serum inhibited neutrophil chemotactic activities of three types of CINCs almost completely. The mutant CINC-3, whose amino-terminal amino acid sequence (SELR) was replaced to AAR, lost chemotactic activity of its own but inhibited that of CINC-1 and CINC-2 potently, and that of CINC-3 weakly. The results indicate that rat CXCR2 on neutrophils is the unique receptor for all three types of CINCs, and CINC-1/-2 and CINC-3 exert different biological activities through the common receptor.
(Copyright 2000 Academic Press.)

0 (CXCL1 protein, human)
0 (Chemokine CXCL1)
0 (Chemokine CXCL2)
0 (Chemokines, CXC)
0 (Chemotactic Factors)
0 (Cxcl1 protein, rat)
0 (Cxcl2 protein, rat)
0 (Cxcl3 protein, rat)
0 (DNA, Complementary)
0 (Growth Substances)
0 (Intercellular Signaling Peptides and Proteins)
0 (Receptors, Interleukin-8B)
59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
SY7Q814VUP (Calcium)

Date Created: 20000908 Date Completed: 20001130 Latest Revision: 20200204

20231215

10.1006/cyto.2000.0739

10975996