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Cloning and recombinant expression of the La RNA-binding protein from Trypanosoma brucei.
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- Author(s): Westermann S;Westermann S; Weber K
- Source:
Biochimica et biophysica acta [Biochim Biophys Acta] 2000 Jul 24; Vol. 1492 (2-3), pp. 483-7.
- Publication Type:
Journal Article
- Language:
English
- Additional Information
- Source:
Publisher: Elsevier Pub. Co Country of Publication: Netherlands NLM ID: 0217513 Publication Model: Print Cited Medium: Print ISSN: 0006-3002 (Print) Linking ISSN: 00063002 NLM ISO Abbreviation: Biochim Biophys Acta Subsets: MEDLINE
- Publication Information:
Original Publication: Amsterdam : Elsevier Pub. Co.
- Subject Terms:
- Abstract:
We report the isolation, cloning and recombinant expression of a Trypanosoma brucei homolog of the La RNA-binding protein. Based on peptide sequence information we have isolated a cDNA clone which encodes a protein of 335 amino acids with a predicted molecular weight of 37.7 kDa. The amino acid sequence fits the domain structure of known La proteins and contains a putative ATP-binding site located in the COOH-terminal domain. The cDNA was expressed as a glutathione S-transferase fusion protein in Escherichia coli, and the recombinant protein displayed RNA-binding activity in an electrophoretic mobility shift assay.
- Accession Number:
0 (Autoantigens)
0 (DNA, Complementary)
0 (DNA, Protozoan)
0 (Protozoan Proteins)
0 (RNA-Binding Proteins)
0 (Recombinant Proteins)
0 (Ribonucleoproteins)
- Publication Date:
Date Created: 20000719 Date Completed: 20001019 Latest Revision: 20231213
- Publication Date:
20240829
- Accession Number:
10.1016/s0167-4781(00)00113-5
- Accession Number:
10899586
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