Expresión génica relacionada con el ciclo celular, apoptosis, sinaptogénesis y diferenciación celular en la diferenciación sexual de la rata.

Genetic expression associated to cell cycle, apoptosis, synaptogenesis and cell differentiation during sex differentiation in rats.

Existen diferencias anatómico-funcionales importantes entre los hipotálamos de las ratas machos y hembras, las cuales son reguladas por esteroides sexuales durante un periodo crítico del desarrollo hipotalámico, especialmente por el estradiol; por ejemplo, en la rata macho, el núcleo dimórfico sexual del área preóptica es seis veces más grande que en la hembra, y en el núcleo arqueado de la hembra son más abundantes las conexiones sinápticas que en los machos. En este estudio se investigaron algunas diferencias entre machos y hembras en la expresión de genes relacionados con la apoptosis, neurogénesis y sinaptogénesis en ratas de 4 h de nacidas, además se evaluó el efecto de la administración temprana de propionato de testosterona (PT) a hembras y tamoxifen (Tx) a machos, sobre el patrón de expresión del grupo de genes referidos, para lo cual se usó un análisis con microarreglos de DNA, combinado con qPCR; se encontraron diferencias en la expresión de los genes en hipotálamos de hembras y machos. En las hembras, hubo una mayor expresión de genes relacionados con la apoptosis: IL-24, Smpd3, Tpa, Pp4, Map3k1, Pge y Naca3; con la diferenciación celular, Neurod2, Zic1 y Epo y con la sinapsis y el control del ciclo celular; Syt7, Tgfbr1, Ptf1a y Cox2. También se muestra que la aplicación de Tx en los machos provocó un patrón de expresión génica similar al de las hembras testigo, mientras que el PT en las hembras no modificó la expresión de genes. [ABSTRACT FROM AUTHOR]

Important anatomical-functional differences are found between hypothalamus of male and female rats which appear and are regulated by sexual steroids during the critical hypothalamic development period. This is especially true of estradiol's involvement. In this study, several genetic differences between male and female rats were assessed. These differences are related to gene expression to apoptosis, neurogenesis and synaptogenesis in four-hour old rats. The effect of early administration of testosterone propionate (TP) to female rats, and tamoxifen (Tx) to male rats on the gene pattern expression was reviewed using DNA microarray analysis combined with qPRC. Gene expression differences were found in female and male hypothalamus. In female rats, there was greater gene expression related to apoptosis: IL-24, Smpd3, Tpa, Pp4, Map3k1, Pge and Naca3; to cell differentiation, Neurod2, Zic1 and Epo; and to synapsis and the control of the cell cycle, Syt7, Tgfbr1, Ptf1a and Cox2. It was also shown that administration of Tx to male rats caused similar genetic expression to that of female rats, while TP given to female rats was not as effective in modifying gene expression. These results clearly show that in the absence of estradiol in female rats, genes favoring cell death are expressed which may explain size differences in certain hypothalamic areas in male and female rats. This may be due too to the fact that in male hypothalamus, certain areas are provided with greater estradiol alpha type receptors and therefore with manifest neuroprotection and other areas with beta receptors where apoptosis predominates. [ABSTRACT FROM AUTHOR]

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