Optimized synthesis of L-sorbose by C(5)-dehydrogenation of D-sorbitol with Gluconobacter oxydans.

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  • Author(s): De Wulf P;De Wulf P; Soetaert W; Vandamme EJ
  • Source:
    Biotechnology and bioengineering [Biotechnol Bioeng] 2000 Aug 05; Vol. 69 (3), pp. 339-43.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Wiley Country of Publication: United States NLM ID: 7502021 Publication Model: Print Cited Medium: Print ISSN: 0006-3592 (Print) Linking ISSN: 00063592 NLM ISO Abbreviation: Biotechnol Bioeng Subsets: MEDLINE
    • Publication Information:
      Publication: <2005->: Hoboken, NJ : Wiley
      Original Publication: New York, Wiley.
    • Subject Terms:
      Gluconobacter oxydans/*metabolism ; Sorbitol/*metabolism ; Sorbose/*metabolism; Ascorbic Acid/biosynthesis ; Ascorbic Acid/economics ; Biomass ; Cell Division/drug effects ; Culture Media/chemistry ; Culture Media/metabolism ; Fermentation ; Gluconobacter oxydans/drug effects ; Gluconobacter oxydans/enzymology ; Gluconobacter oxydans/genetics ; Hydrogen-Ion Concentration ; Hydrogenation ; L-Iditol 2-Dehydrogenase/metabolism ; Mutation ; Sorbitol/chemistry ; Sorbitol/pharmacology ; Sorbose/chemistry ; Stereoisomerism ; Time Factors
    • Abstract:
      The optimization of L-sorbose synthesis by regiospecific dehydrogenation of D-sorbitol using Gluconobacter oxydans is reported. The current L-sorbose production processes that are based on G. oxydans and other bacterial strains are suboptimal as to yield and rate of L-sorbose synthesis. One reason for these problems is the toxicity that is induced by the substrate D-sorbitol when used in concentrations of >10% (w/v). This phenomenon significantly limits the potentials of L-sorbose production from an industrial point of view. The goal of this study was to develop a fast production process that yields L-sorbose in stoichiometric amounts starting from D-sorbitol concentrations that exceed 10% (w/v). A gradual improvement of the inoculum build-up procedure, culture medium composition, and process parameters ultimately led to a theoretically maximal L-sorbose productivity (200 g L(-1) of L-sorbose from 200 g L(-1) of D-sorbitol in 28 h of fermentation) using a Gluconobacter oxydans mutant strain that was selected under conditions of substrate inhibition. Because the D-sorbitol/L&HYPHEN;sorbose bioconversion is used to mass-produce vitamin C, the procedure reported here will contribute to a more efficient and more economic synthesis of vitamin C.
      (Copyright 2000 John Wiley & Sons, Inc.)
    • Accession Number:
      0 (Culture Media)
      506T60A25R (Sorbitol)
      EC 1.1.1.14 (L-Iditol 2-Dehydrogenase)
      NV2001607Y (Sorbose)
      PQ6CK8PD0R (Ascorbic Acid)
    • Publication Date:
      Date Created: 20000622 Date Completed: 20000823 Latest Revision: 20190921
    • Publication Date:
      20221213
    • Accession Number:
      10.1002/1097-0290(20000805)69:3<339::aid-bit12>3.0.co;2-e
    • Accession Number:
      10861414