Three-Dimensional Cell Culture Assays: Are They More Predictive of In Vivo Efficacy than 2D Monolayer Cell-Based Assays?

Information about topics discussed at a roundtable on three-dimensional (3D) cell culture assays moderated by Sitta Sittampalam, senior advisor to the director of the U.S. National Institutes of Health (NIH) National Center for Advancing Translational Sciences (NCATS), is presented. Topics include the comparison between the use of 3D cell cultures and 2D monolayer cell-based assays in industry and academia, and the predictive value and in vivo efficacy and toxicity of 3D assays compared to 2D.