CDP/Cut DNA binding activity is down-modulated in granulocytes, macrophages and erythrocytes but remains elevated in differentiating megakaryocytes.

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  • Additional Information
    • Source:
      Publisher: Nature Publishing Group, Specialist Journals Country of Publication: England NLM ID: 8704895 Publication Model: Print Cited Medium: Print ISSN: 0887-6924 (Print) Linking ISSN: 08876924 NLM ISO Abbreviation: Leukemia Subsets: MEDLINE
    • Publication Information:
      Publication: 2000- : London : Nature Publishing Group, Specialist Journals
      Original Publication: [Baltimore, Md.] : Williams & Wilkins, [c1987-
    • Subject Terms:
      Erythrocytes/*metabolism ; Granulocytes/*metabolism ; Homeodomain Proteins/*metabolism ; Macrophages/*metabolism ; Megakaryocytes/*metabolism ; Nuclear Proteins/*metabolism ; Repressor Proteins/*metabolism; Animals ; Bone Marrow Cells/cytology ; Cell Differentiation/drug effects ; Drosophila Proteins ; Drosophila melanogaster ; Hematopoietic Stem Cells/cytology ; Hematopoietic Stem Cells/metabolism ; Humans ; K562 Cells ; Leukemia, Erythroblastic, Acute ; Macrophages/cytology ; Mice ; Mice, Inbred Strains ; Nerve Tissue Proteins ; Tetradecanoylphorbol Acetate/pharmacology ; Transcription Factors ; Tumor Cells, Cultured
    • Abstract:
      DNA binding by the CCAAT-displacement protein, the mammalian homologue of the Drosophila melanogaster Cut protein, was previously found to increase sharply in S phase, suggesting a role for CDP/Cut in cell cycle progression. Genetic studies in Drosophila indicated that cut plays an important role in cell-type specification in several tissues. In the present study, we have investigated CDP/Cut expression and activity in a panel of multipotent hematopoietic cell lines that can be induced to differentiate in vitro into distinct cell types. While CDP/Cut DNA binding activity declined in the pathways leading to macrophages, granulocytes and erythrocytes, it remained elevated in megakaryocytes. CDP/Cut was also highly expressed in primary megakaryocytes isolated from mouse, and some DNA binding activity could be detected. Altogether, these results raise the possibility that CDP/Cut may be a determinant of cell type identity downstream of the myelo-erythroid precursor cell. Another possibility, which does not exclude a role in lineage identity, is that CDP/Cut activity in megakaryocytes is linked to endomitosis. Indeed, elevated CDP/Cut activity in differentiating megakaryocytes and during the S phase of the cell cycle suggests that it may be required for DNA replication.
    • Grant Information:
      K08 HL03498 United States HL NHLBI NIH HHS; R01 CA31615 United States CA NCI NIH HHS; R01 DK49855 United States DK NIDDK NIH HHS
    • Accession Number:
      0 (CUX1 protein, human)
      0 (Cux1 protein, mouse)
      0 (Drosophila Proteins)
      0 (Homeodomain Proteins)
      0 (Nerve Tissue Proteins)
      0 (Nuclear Proteins)
      0 (Repressor Proteins)
      0 (Transcription Factors)
      0 (ct protein, Drosophila)
      NI40JAQ945 (Tetradecanoylphorbol Acetate)
    • Publication Date:
      Date Created: 20000510 Date Completed: 20000519 Latest Revision: 20191120
    • Publication Date:
      20240829
    • Accession Number:
      10.1038/sj.leu.2401764
    • Accession Number:
      10803519