A rapid and specific radioimmunoassay for methotrexate.

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  • Author(s): Raso V; Schreiber R
  • Source:
    Cancer research [Cancer Res] 1975 Jun; Vol. 35 (6), pp. 1407-10.
  • Publication Type:
    Journal Article; Research Support, U.S. Gov't, P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: American Association for Cancer Research Country of Publication: United States NLM ID: 2984705R Publication Model: Print Cited Medium: Print ISSN: 0008-5472 (Print) Linking ISSN: 00085472 NLM ISO Abbreviation: Cancer Res Subsets: MEDLINE
    • Publication Information:
      Publication: Baltimore, Md. : American Association for Cancer Research
      Original Publication: Chicago [etc.]
    • Subject Terms:
      Radioimmunoassay*; Methotrexate/*analysis; Animals ; Antibody Specificity ; Binding Sites, Antibody ; Humans ; Leucovorin/pharmacology ; Methotrexate/cerebrospinal fluid ; Methotrexate/metabolism ; Methotrexate/urine ; Rabbits/immunology
    • Abstract:
      A sensitive and precise radioimmunoassay for methotrexate has been developed using antibody induced in rabbits, tritium-labeled methotrexate, and a nitrocellulose membrane separation technique. Antibody specificity was characterized by comparing the effectiveness of various related compounds to displace labeled methotrexate from the antibody-binding site. Assay of serum samples from persons receiving the drug was rapid and easy to perform. In a pharmacokinetic study of methotrexate, corresponding results were obtained when measurements were made by either enzymic assay or by radioimmunoassay. Drug concentrations could also be monitored in the cerebrospinal fluid and urine of patients on high-dose methotrexate therapy followed by citrovorum factor rescue. The system measured a little as 0.1 to 1 pmole of methotrexate, depending upon the antiserum used, and naturally occurring folates did not interfere with these determinations.
    • Accession Number:
      Q573I9DVLP (Leucovorin)
      YL5FZ2Y5U1 (Methotrexate)
    • Publication Date:
      Date Created: 19750601 Date Completed: 19750904 Latest Revision: 20131121
    • Publication Date:
      20231215
    • Accession Number:
      1079473