Calibration of disk diffusion antibiotic susceptibility testing: species-related trovafloxacin interpretive zone breakpoints and selection of disk potency.

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  • Additional Information
    • Source:
      Publisher: Informa Healthcare Country of Publication: England NLM ID: 0215333 Publication Model: Print Cited Medium: Print ISSN: 0036-5548 (Print) Linking ISSN: 00365548 NLM ISO Abbreviation: Scand J Infect Dis Subsets: MEDLINE
    • Publication Information:
      Publication: 2007-2014 : London : Informa Healthcare
      Original Publication: Stockholm, Society for the Publication of Acta medica Scandinavica.
    • Subject Terms:
      Fluoroquinolones*; Anti-Infective Agents/*pharmacology ; Microbial Sensitivity Tests/*methods ; Naphthyridines/*pharmacology; Calibration ; Diffusion ; Humans ; Microbial Sensitivity Tests/standards ; Quality Control ; Regression Analysis
    • Abstract:
      International comparisons of antibiotic susceptibility require the use of common minimum inhibitory concentration (MIC) limits. Disk diffusion test results are not directly suitable for such comparisons, since different standards are often used and zone breakpoints issued might reflect different MIC limits. We have used single strain regression analysis (SRA) for the calibration of the disk test, both according to species and individual laboratory, and for quality control of trovafloxacin disk diffusion tests in 5 laboratories in Sweden. Preliminary controls using histogram analysis including subtraction histograms of reference strains revealed marked differences between different laboratories. SRA was performed on 4 reference strains, S. aureus, E. faecalis, E. coli and P. aeruginosa, using disks containing 1, 3, 10, 30 and 100 microg trovafloxacin. The results using SRA showed a difference between laboratories using Biodisk PDM medium, which produced smaller zones, and those using Oxoid IsoSensitest. Species-related regression lines for laboratories using either medium were calculated and corresponding interpretive zone breakpoints determined for MIC limits. Rational criteria for the selection of a suitable disk content of an antibiotic were also defined and applied to trovafloxacin. The 10 microg disk selected by NCCLS (National Committee for Clinical Laboratory Standards) proved optimal.
    • Accession Number:
      0 (Anti-Infective Agents)
      0 (Fluoroquinolones)
      0 (Naphthyridines)
      9F388J00UK (trovafloxacin)
    • Publication Date:
      Date Created: 20000219 Date Completed: 20000302 Latest Revision: 20190826
    • Publication Date:
      20231215
    • Accession Number:
      10.1080/00365549950164463
    • Accession Number:
      10680988