Intracellular mRNA cleavage induced through activation of RNase P by nuclease-resistant external guide sequences.

Publisher: Nature America Publishing Country of Publication: United States NLM ID: 9604648 Publication Model: Print Cited Medium: Print ISSN: 1087-0156 (Print) Linking ISSN: 10870156 NLM ISO Abbreviation: Nat Biotechnol Subsets: MEDLINE

Publication: New York Ny : Nature America Publishing
Original Publication: New York, NY : Nature Pub. Co., [1996-

Endoribonucleases/*metabolism ; RNA, Catalytic/*metabolism ; RNA, Messenger/*metabolism; 3' Untranslated Regions/genetics ; Blotting, Western ; Down-Regulation ; Enzyme Activation ; Humans ; Isoenzymes/genetics ; Isoenzymes/metabolism ; Nucleic Acid Conformation ; Oligoribonucleotides/chemistry ; Oligoribonucleotides/genetics ; Phosphatidylethanolamines/metabolism ; Protein Kinase C/genetics ; Protein Kinase C/metabolism ; Protein Kinase C-alpha ; Proto-Oncogene Proteins c-bcl-2/analysis ; Proto-Oncogene Proteins c-bcl-2/genetics ; RNA Processing, Post-Transcriptional/genetics ; RNA, Antisense/chemistry ; RNA, Antisense/genetics ; RNA, Antisense/physiology ; RNA, Messenger/genetics ; RNA, Transfer/chemistry ; RNA, Transfer/genetics ; RNA, Transfer/metabolism ; Ribonuclease H/metabolism ; Ribonuclease P ; Substrate Specificity ; Transfection/methods ; Tumor Cells, Cultured ; Urinary Bladder Neoplasms/enzymology ; Urinary Bladder Neoplasms/genetics ; Urinary Bladder Neoplasms/pathology ; bcl-X Protein ; RNA, Small Untranslated

Most antisense oligonucleotide experiments are performed with molecules containing RNase H-competent backbones. However, RNase H may cleave nontargeted mRNAs bound to only partially complementary oligonucleotides. Decreasing such "irrelevant cleavage" would be of critical importance to the ability of the antisense biotechnology to provide accurate assessment of gene function. RNase P is a ubiquitous endogenous cellular ribozyme whose function is to cleave the 5' terminus of precursor tRNAs to generate the mature tRNA. To recruit RNase P, complementary oligonucleotides called external guide sequences (EGS), which mimic structural features of precursor tRNA, were incorporated into an antisense 2'-O-methyl oligoribonucleotide targeted to the 3' region of the PKC-alpha mRNA. In T24 human bladder carcinoma cells, these EGSs, but not control sequences, were highly effective in downregulating PKC-alpha protein and mRNA expression. Furthermore, the downregulation is dependent on the presence of, and base sequence in, the T-loop. Similar observations were made with an EGS targeted to the bcl-xL mRNA.

0 (3' Untranslated Regions)
0 (BCL2L1 protein, human)
0 (Isoenzymes)
0 (Oligoribonucleotides)
0 (Phosphatidylethanolamines)
0 (Proto-Oncogene Proteins c-bcl-2)
0 (RNA, Antisense)
0 (RNA, Catalytic)
0 (RNA, Messenger)
0 (bcl-X Protein)
76391-83-8 (1,2-dielaidoylphosphatidylethanolamine)
9014-25-9 (RNA, Transfer)
EC 2.7.11.1 (protein kinase C zeta)
EC 2.7.11.13 (PRKCA protein, human)
EC 2.7.11.13 (Protein Kinase C)
EC 2.7.11.13 (Protein Kinase C-alpha)
EC 3.1.- (Endoribonucleases)
EC 3.1.26.4 (Ribonuclease H)
EC 3.1.26.5 (RPP14 protein, human)
EC 3.1.26.5 (Ribonuclease P)
0 (RNA, Small Untranslated)

Date Created: 20000114 Date Completed: 20000229 Latest Revision: 20231130

20240829

10.1038/71924

10625392