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Biochemical and molecular characterization of a succinate semialdehyde dehydrogenase involved in the catabolism of 4-hydroxybutyric acid in Ralstonia eutropha.
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- Additional Information
- Source:
Publisher: Oxford University Press Country of Publication: England NLM ID: 7705721 Publication Model: Print Cited Medium: Print ISSN: 0378-1097 (Print) Linking ISSN: 03781097 NLM ISO Abbreviation: FEMS Microbiol Lett Subsets: MEDLINE
- Publication Information:
Publication: 2015- : Oxford Oxford University Press
Original Publication: Amsterdam, Published by Elsevier/North Holland on behalf of the Federation of European Microbiological Societies.
- Subject Terms:
- Abstract:
A succinate semialdehyde dehydrogenase gene (gabD) was identified to be disrupted in a transposon-induced mutant of Ralstonia eutropha exhibiting the phenotype 4-hydroxybutyric acid-leaky. The native gabD gene was cloned by colony hybridization using a homologous gabD-specific DNA probe. DNA sequencing revealed an 1452-bp open reading frame, and the deduced amino acid sequence showed strong similarities to NADP(+)-dependent succinate semialdehyde dehydrogenases from Escherichia coli, Rhizobium sp., Homo sapiens and Rattus norvegicus. The gabD gene was heterologously expressed in a recombinant E. coli strain harboring plasmid pSK::EE6.8. Similar to the molecular organization of the gab cluster in E. coli, additional genes encoding enzymes for the degradation of gamma-aminobutyrate are closely related to gabD in R. eutropha. Enzymatic studies indicated the existence of a second NAD(+)-dependent succinate semialdehyde dehydrogenase in R. eutropha.
- Molecular Sequence:
GENBANK AF175740
- Accession Number:
0 (DNA Transposable Elements)
0 (Hydroxybutyrates)
30IW36W5B2 (4-hydroxybutyric acid)
EC 1.2.- (Aldehyde Oxidoreductases)
EC 1.2.1.24 (Succinate-Semialdehyde Dehydrogenase)
- Publication Date:
Date Created: 19991124 Date Completed: 20000203 Latest Revision: 20181130
- Publication Date:
20240829
- Accession Number:
10.1111/j.1574-6968.1999.tb08827.x
- Accession Number:
10564790
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