Regulated expression and subcellular localization of syndecan heparan sulfate proteoglycans and the syndecan-binding protein CASK/LIN-2 during rat brain development.

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  • Author(s): Hsueh YP;Hsueh YP; Sheng M
  • Source:
    The Journal of neuroscience : the official journal of the Society for Neuroscience [J Neurosci] 1999 Sep 01; Vol. 19 (17), pp. 7415-25.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Society for Neuroscience Country of Publication: United States NLM ID: 8102140 Publication Model: Print Cited Medium: Internet ISSN: 1529-2401 (Electronic) Linking ISSN: 02706474 NLM ISO Abbreviation: J Neurosci Subsets: MEDLINE
    • Publication Information:
      Publication: Washington, DC : Society for Neuroscience
      Original Publication: [Baltimore, Md.] : The Society, c1981-
    • Subject Terms:
      Gene Expression Regulation, Developmental*; Aging/*metabolism ; Brain/*metabolism ; Heparan Sulfate Proteoglycans/*genetics ; Membrane Glycoproteins/*genetics ; Neurons/*metabolism ; Proteoglycans/*genetics; Amino Acid Sequence ; Animals ; Brain/embryology ; Brain/growth & development ; COS Cells ; Embryonic and Fetal Development ; Immunohistochemistry ; Membrane Glycoproteins/chemistry ; Molecular Sequence Data ; Proteoglycans/chemistry ; RNA, Messenger/genetics ; Rats ; Recombinant Proteins/biosynthesis ; Sequence Alignment ; Sequence Homology, Amino Acid ; Subcellular Fractions/metabolism ; Synapses/metabolism ; Syndecan-1 ; Syndecan-2 ; Syndecan-3 ; Syndecan-4 ; Syndecans ; Transcription, Genetic ; Transfection
    • Abstract:
      The syndecan family of cell surface heparan sulfate proteoglycans interacts via their cytoplasmic C-terminal tail with the PDZ domain of CASK/LIN-2, a membrane-associated guanylate kinase homolog. The syndecan-CASK interaction may be involved in intercellular signaling and/or cell adhesion. Here we show that syndecan-1 to syndecan-4 have distinctive mRNA distributions in adult rat brain by in situ hybridization, with syndecan-2 and -3 being the major syndecans expressed in neurons of the forebrain. At the protein level, syndecan-2 and -3 are differentially localized within neurons; syndecan-3 is concentrated in axons, whereas syndecan-2 is localized in synapses. The synaptic accumulation of syndecan-2 occurs late in synapse development. CASK is a cytoplasmic-binding partner for syndecans, and its subcellular distribution changes strikingly during development, shifting from a primarily axonal distribution in the first 2 postnatal weeks to a somatodendritic distribution in adult brain. This change in CASK distribution correlates temporally and spatially with the expression patterns of syndecan-3 and -2, consistent with the association of both of these syndecans with CASK in vivo. In support of this, we were able to coimmunoprecipitate a complex of CASK and syndecan-3 from brain extracts. Our results indicate that specific syndecans are differentially expressed in various cell types of the brain and are targeted to distinct subcellular compartments in neurons, where they may serve specialized functions. Moreover, CASK is appropriately expressed and localized to interact with both syndecan-2 and -3 in different compartments of the neuron throughout postnatal development.
    • References:
      Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):13683-8. (PMID: 9391086)
      J Biol Chem. 1999 Jan 1;274(1):532-6. (PMID: 9867876)
      Cell. 1995 Nov 3;83(3):357-60. (PMID: 8521464)
      Biochem J. 1997 Oct 1;327 ( Pt 1):1-16. (PMID: 9355727)
      J Cell Biol. 1998 Jul 13;142(1):139-51. (PMID: 9660869)
      J Cell Biochem. 1996 Jun 15;61(4):578-84. (PMID: 8806081)
      J Cell Biol. 1995 Jul;130(1):127-35. (PMID: 7540614)
      J Cell Biol. 1998 Jul 13;142(1):129-38. (PMID: 9660868)
      J Neurosci. 1999 Feb 15;19(4):1226-35. (PMID: 9952400)
      J Cell Biol. 1983 May;96(5):1374-88. (PMID: 6404912)
      J Neurosci. 1998 Feb 15;18(4):1217-29. (PMID: 9454832)
      Cell. 1998 Sep 18;94(6):761-71. (PMID: 9753323)
      J Neurochem. 1998 Oct;71(4):1339-47. (PMID: 9751164)
      J Biol Chem. 1994 Apr 29;269(17):12999-3004. (PMID: 8175719)
      J Biol Chem. 1997 Jan 31;272(5):2873-9. (PMID: 9006931)
      Cell. 1998 Sep 18;94(6):773-82. (PMID: 9753324)
      J Biol Chem. 1997 Mar 28;272(13):8133-6. (PMID: 9079625)
      Neurosci Lett. 1995 May 19;191(1-2):39-42. (PMID: 7659286)
      Annu Rev Cell Biol. 1992;8:365-93. (PMID: 1335744)
      Neuron. 1994 Oct;13(4):823-35. (PMID: 7946331)
      J Cell Biol. 1992 Apr;117(1):191-201. (PMID: 1556152)
      Mech Dev. 1997 Apr;63(1):127-30. (PMID: 9178262)
      Mol Biol Cell. 1994 Jul;5(7):797-805. (PMID: 7812048)
      Neuron. 1997 Nov;19(5):1007-16. (PMID: 9390515)
      J Biol Chem. 1997 Jun 6;272(23):14713-20. (PMID: 9169435)
      FASEB J. 1993 Aug;7(11):1023-30. (PMID: 8370471)
      Development. 1999 Feb;126(4):781-91. (PMID: 9895325)
      J Neurosci. 1996 Apr 15;16(8):2488-94. (PMID: 8786425)
      Dev Suppl. 1993;:205-12. (PMID: 8049475)
      J Neurosci. 1997 Jun 15;17(12):4734-43. (PMID: 9169533)
      Neuron. 1998 Dec;21(6):1453-63. (PMID: 9883737)
      Science. 1992 Jul 3;257(5066):50-6. (PMID: 1621094)
      Development. 1996 Jan;122(1):97-111. (PMID: 8565857)
      J Cell Biol. 1999 Feb 8;144(3):575-86. (PMID: 9971750)
      Curr Opin Cell Biol. 1998 Oct;10(5):620-8. (PMID: 9818173)
    • Grant Information:
      R01 NS035050 United States NS NINDS NIH HHS; NS35050 United States NS NINDS NIH HHS
    • Accession Number:
      0 (Heparan Sulfate Proteoglycans)
      0 (Membrane Glycoproteins)
      0 (Proteoglycans)
      0 (RNA, Messenger)
      0 (Recombinant Proteins)
      0 (Sdc1 protein, rat)
      0 (Sdc2 protein, rat)
      0 (Sdc3 protein, rat)
      0 (Sdc4 protein, rat)
      0 (Syndecan-1)
      0 (Syndecan-3)
      0 (Syndecan-4)
      0 (Syndecans)
      149769-25-5 (Syndecan-2)
    • Publication Date:
      Date Created: 19990825 Date Completed: 19990923 Latest Revision: 20240421
    • Publication Date:
      20240421
    • Accession Number:
      PMC6782500
    • Accession Number:
      10460248