Pulmonary artery endothelium-dependent vasodilation is impaired in a chicken model of pulmonary hypertension.

Publisher: American Physiological Society Country of Publication: United States NLM ID: 0370511 Publication Model: Print Cited Medium: Print ISSN: 0002-9513 (Print) Linking ISSN: 00029513 NLM ISO Abbreviation: Am J Physiol Subsets: MEDLINE

Publication: Bethesda, MD : American Physiological Society
Original Publication: Washington [etc.] American Physiological Society.

Disease Models, Animal*; Chickens/*physiology ; Endothelium, Vascular/*physiopathology ; Hypertension, Pulmonary/*physiopathology ; Pulmonary Artery/*physiopathology ; Vasodilation/*physiology; Acetylcholine/pharmacology ; Animals ; Arginine/pharmacology ; Calcimycin/pharmacology ; Chickens/classification ; Endothelium, Vascular/drug effects ; Enzyme Inhibitors/pharmacology ; Ionophores/pharmacology ; Male ; NG-Nitroarginine Methyl Ester/pharmacology ; Nitroprusside/pharmacology ; Papaverine/pharmacology ; Pulmonary Artery/drug effects ; Vasodilation/drug effects ; Vasodilator Agents/pharmacology

Among chicken strains, broilers are prone to pulmonary hypertension, whereas Leghorns are not. Relaxations to endothelium-dependent (ACh, A23187) and endothelium-independent [sodium nitroprusside (SNP), papaverine (PPV)] vasodilators were compared in preconstricted pulmonary artery (PA) rings from these chicken strains. ACh (10(-7), 10(-6), and 10(-5) M)- and A23187 (10(-6) and 10(-5.5) M)-induced relaxations were smaller (P < 0.05) in broilers than Leghorns. N(G)-nitro-L-arginine methyl ester (10(-3.5) M) caused similar reductions in ACh-induced relaxations in both strains. L-Arginine (10(-4) M) enhanced ACh-induced relaxations more in broilers than Leghorns. Relaxations to 10(-10)-10(-6) M SNP did not differ between strains, but were greater (P < 0.05) in broilers than Leghorns at higher concentrations (10(-5) and 10(-4) M). PPV (10(-4) M)- and SNP (10(-4) M)-induced maximal relaxations were greater in broilers than in Leghorns (176.2 +/- 14.7 vs. 120.9 +/- 14.7% and 201.3 +/- 7.8 vs. 171.2 +/- 10.7%, respectively, P < 0.05). Broiler PA rings appear to have increased intrinsic tone and reduced endothelium-derived nitric oxide activity, both of which may contribute to the susceptibility of broiler chickens to pulmonary hypertension.

0 (Enzyme Inhibitors)
0 (Ionophores)
0 (Vasodilator Agents)
169D1260KM (Nitroprusside)
37H9VM9WZL (Calcimycin)
94ZLA3W45F (Arginine)
DAA13NKG2Q (Papaverine)
N9YNS0M02X (Acetylcholine)
V55S2QJN2X (NG-Nitroarginine Methyl Ester)

Date Created: 19990717 Date Completed: 19990830 Latest Revision: 20181106

20221213

10.1152/ajpregu.1999.277.1.R190

10409273