Apolipoprotein E (ApoE), a Bmp-2 (bone morphogenetic protein) upregulated gene in mesenchymal progenitors (C3H10T1/2), is highly expressed in murine embryonic development.

Publisher: Ios Press Country of Publication: Netherlands NLM ID: 8807441 Publication Model: Print Cited Medium: Print ISSN: 0951-6433 (Print) Linking ISSN: 09516433 NLM ISO Abbreviation: Biofactors Subsets: MEDLINE

Publication: Amsterdam : Ios Press
Original Publication: Oxford ; Washington, DC : Published for the International Union of Biochemistry by IRL Press, [c1988-

Gene Expression Regulation, Developmental*; Apolipoproteins E/*genetics ; Bone Morphogenetic Proteins/*physiology ; Embryo, Mammalian/*metabolism ; Mesoderm/*cytology ; Stem Cells/*metabolism; Animals ; Apolipoproteins E/metabolism ; Bone Morphogenetic Proteins/genetics ; Cell Differentiation ; Cell Line ; Cell Lineage ; Cells, Cultured ; Cloning, Molecular ; Embryo, Mammalian/cytology ; Embryonic and Fetal Development ; In Situ Hybridization ; Mesoderm/metabolism ; Mice ; Mice, Inbred Strains ; Neural Crest/cytology ; Neural Crest/embryology ; Neural Crest/metabolism ; Osteoblasts/cytology ; Osteoblasts/metabolism ; Recombinant Proteins/metabolism ; Transfection ; Up-Regulation

Apolipoprotein E (ApoE) was identified as upregulated by Bmp-2 (bone morphogenetic protein-2) in the murine mesenchymal progenitor cell line C3H10T1/2 by a subtractive cloning strategy. Expression of recombinant Bmps in mesenchymal C3H10T1/2 progenitors results in the differentiation into the osteogenic, the chondrogenic, and the adipogenic lineage. In addition, ApoE is also expressed in primary osteoblasts isolated from murine calvariae late in the in vitro osteoblast developmental sequence. To infer possible roles of ApoE in organogenesis and tissue differentiation, ApoE expression during mouse embryonic development was analyzed in murine midgestation and late embryonic development by in situ hybridization. ApoE is highly expressed at many sites of organ development (liver, brain, heart, eye, lung), probably in a subset of neural crest cells and ectodermal derivatives suggestive for important functions of ApoE during embryonic differentiation and organ development.

0 (Apolipoproteins E)
0 (Bone Morphogenetic Proteins)
0 (Recombinant Proteins)

Date Created: 19990430 Date Completed: 19990614 Latest Revision: 20191024

20240829

10.1002/biof.5520090103

10221153