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Isolation of plant-growth-promoting rhizobacteria from rhizospheric soil of halophytes and their impact on maize ( Zea mays L.) under induced soil salinity.
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- Author(s): Ullah, Sami; Bano, Asghari
- Source:
Canadian Journal of Microbiology; Apr2015, Vol. 61 Issue 4, p307-313, 7p, 5 Charts, 8 Graphs
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- Abstract:
The present investigation was aimed to scrutinize the salt tolerance potential of plant-growth-promoting rhizobacteria (PGPR) isolated from rhizospheric soil of selected halophytes ( Atriplex leucoclada, Haloxylon salicornicum, Lespedeza bicolor, Suaeda fruticosa, and Salicornica virginica) collected from high-saline fields (electrical conductivity 4.3-5.5) of District Mardan, Pakistan. Five PGPR strains were identified using 16S rRNA amplification and sequence analysis. Bacillus sp., isolated from rhizospheric soil of Atriplex leucoclada, and Arthrobacter pascens, isolated from rhizospheric soil of Suaeda fruticosa, are active phosphate solubilizers and bacteriocin and siderophore producers; hence, their inoculation and co-inoculation on maize ('Rakaposhi') under induced salinity stress enhanced shoot and root length and shoot and root fresh and dry mass. The accumulation of osmolytes, including sugar and proline, and the elevation of antioxidant enzymes activity, including superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase, were enhanced in the maize variety when inoculated and co-inoculated with Bacillus sp. and Arthrobacter pascens. The PGPR ( Bacillus sp. and A. pascens) isolated from the rhizosphere of the mentioned halophytes species showed reliability in growth promotion of maize crop in all the physiological parameters; hence, they can be used as bio-inoculants for the plants growing under salt stress. [ABSTRACT FROM AUTHOR]
- Abstract:
La présente étude avait pour objectif d'examiner le potentiel de tolérance au sel de rhizobactéries favorisant la croissance végétale (PGPR) isolées de sols rhizosphériques associés à des halophytes définis ( Atriplex leucoclada, Haloxylon salicornicum, Lespedeza bicolor, Suaeda fruticosa et Salicornica virginica) prélevés de terres à salinité élevée du district de Mardan (CE de 4,3 à 5,5) au Pakistan. On a identifié les cinq souches de PGPR par amplification et analyse de la séquence de l'ARNr 16S. Une espèce de Bacillus isolée du sol rhizosphérique d' Atriplex leucoclada et Arthrobacter pascens isolés de sol rhizosphérique de Suaeda fruticosa sont des solubilisants de phosphates et des producteurs de bactériocines et de sidérophores. Ainsi, leur inoculation et co-inoculation sur une variété de maïs (Rakaposhi) dans des conditions de stress salin a haussé la longueur et les poids frais et sec des tiges et des racines. On a mesuré une hausse de l'accumulation d'osmolytes, dont le sucre et la proline, et de l'activité enzymatique antioxydante, dont celle de la superoxyde dismutase, de la catalase et de l'ascorbate peroxydase, chez les maïs inoculés ou co-inoculés avec Bacillus sp. et Arthrobacter pascens. Les PGPR ( Bacillus sp. et A. pascens) isolées de la rhizosphère des espèces d'halophytes énoncées précédemment ont démontré la faculté de favoriser la croissance de cultures de maïs sur la foi de tous les paramètres physiologiques, et pourraient donc faire office de bio-inoculants pour les plantes devant composer avec un stress salin. [Traduit par la Rédaction] [ABSTRACT FROM AUTHOR]
- Abstract:
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