Redundant systems of phosphatidic acid biosynthesis via acylation of glycerol-3-phosphate or dihydroxyacetone phosphate in the yeast Saccharomyces cerevisiae.

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  • Additional Information
    • Source:
      Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 2985120R Publication Model: Print Cited Medium: Print ISSN: 0021-9193 (Print) Linking ISSN: 00219193 NLM ISO Abbreviation: J Bacteriol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, DC : American Society for Microbiology
    • Subject Terms:
    • Abstract:
      In the yeast Saccharomyces cerevisiae lipid particles harbor two acyltransferases, Gat1p and Slc1p, which catalyze subsequent steps of acylation required for the formation of phosphatidic acid. Both enzymes are also components of the endoplasmic reticulum, but this compartment contains additional acyltransferase(s) involved in the biosynthesis of phosphatidic acid (K. Athenstaedt and G. Daum, J. Bacteriol. 179:7611-7616, 1997). Using the gat1 mutant strain TTA1, we show here that Gat1p present in both subcellular fractions accepts glycerol-3-phosphate and dihydroxyacetone phosphate as a substrate. Similarly, the additional acyltransferase(s) present in the endoplasmic reticulum can acylate both precursors. In contrast, yeast mitochondria harbor an enzyme(s) that significantly prefers dihydroxyacetone phosphate as a substrate for acylation, suggesting that at least one additional independent acyltransferase is present in this organelle. Surprisingly, enzymatic activity of 1-acyldihydroxyacetone phosphate reductase, which is required for the conversion of 1-acyldihydroxyacetone phosphate to 1-acylglycerol-3-phosphate (lysophosphatidic acid), is detectable only in lipid particles and the endoplasmic reticulum and not in mitochondria. In vivo labeling of wild-type cells with [2-3H, U-14C]glycerol revealed that both glycerol-3-phosphate and dihydroxyacetone phosphate can be incorporated as a backbone of glycerolipids. In the gat1 mutant and the 1-acylglycerol-3-phosphate acyltransferase slc1 mutant, the dihydroxyacetone phosphate pathway of phosphatidic acid biosynthesis is slightly preferred as compared to the wild type. Thus, mutations of the major acyltransferases Gat1p and Slc1p lead to an increased contribution of mitochondrial acyltransferase(s) to glycerolipid synthesis due to their substrate preference for dihydroxyacetone phosphate.
    • References:
      J Biol Chem. 1993 Oct 15;268(29):22156-63. (PMID: 8408076)
      Methods Enzymol. 1991;194:3-21. (PMID: 2005794)
      Biochim Biophys Acta. 1995 Mar 22;1234(2):214-20. (PMID: 7696296)
      J Bacteriol. 1992 Sep;174(17):5702-10. (PMID: 1512203)
      Yeast. 1995 May;11(6):493-536. (PMID: 7645343)
      Prog Lipid Res. 1995;34(4):343-64. (PMID: 8685243)
      Biochim Biophys Acta. 1997 Sep 4;1348(1-2):27-34. (PMID: 9370313)
      J Bacteriol. 1997 Dec;179(24):7611-6. (PMID: 9401016)
      Mol Biol Cell. 1998 Feb;9(2):375-86. (PMID: 9450962)
      J Biol Chem. 1951 Nov;193(1):265-75. (PMID: 14907713)
      J Biol Chem. 1957 May;226(1):497-509. (PMID: 13428781)
      Nature. 1970 Aug 15;227(5259):680-5. (PMID: 5432063)
      Biochim Biophys Acta. 1970 Dec 15;218(3):431-40. (PMID: 5499200)
      Annu Rev Biochem. 1974;43(0):243-77. (PMID: 4604904)
      J Bacteriol. 1978 Mar;133(3):1368-76. (PMID: 25265)
      Biochim Biophys Acta. 1978 Jul 25;530(1):78-90. (PMID: 356890)
      Biochem J. 1979 Sep 15;182(3):751-62. (PMID: 518562)
      J Biol Chem. 1980 Sep 10;255(17):8289-95. (PMID: 7410364)
      J Biol Chem. 1982 Nov 10;257(21):13028-33. (PMID: 6290489)
      Methods Enzymol. 1983;96:192-205. (PMID: 6228704)
      J Bacteriol. 1986 Mar;165(3):901-10. (PMID: 3005242)
      J Biol Chem. 1986 Jul 15;261(20):9144-9. (PMID: 3522586)
      J Biol Chem. 1987 Oct 25;262(30):14563-70. (PMID: 2822695)
      J Biol Chem. 1991 Feb 25;266(6):3586-93. (PMID: 1995619)
      J Bacteriol. 1991 Mar;173(6):2026-34. (PMID: 2002005)
      Yeast. 1994 Nov;10(11):1421-8. (PMID: 7871881)
    • Accession Number:
      0 (Carbon Radioisotopes)
      0 (Fungal Proteins)
      0 (Glycerophosphates)
      0 (Phosphatidic Acids)
      0 (Phospholipids)
      0 (Saccharomyces cerevisiae Proteins)
      10028-17-8 (Tritium)
      57-04-5 (Dihydroxyacetone Phosphate)
      9NTI6P3O4X (alpha-glycerophosphoric acid)
      EC 2.3.- (Acyltransferases)
      EC 2.3.1.15 (Glycerol-3-Phosphate O-Acyltransferase)
      EC 3.6.4.2 (Dyneins)
      EC 3.6.4.2 (SLC1 protein, S cerevisiae)
      PDC6A3C0OX (Glycerol)
    • Publication Date:
      Date Created: 19990227 Date Completed: 19990407 Latest Revision: 20200724
    • Publication Date:
      20231215
    • Accession Number:
      PMC93534
    • Accession Number:
      10.1128/JB.181.5.1458-1463.1999
    • Accession Number:
      10049376