Description of Cohnella rhizoplanae sp. nov., isolated from the root surface of soybean (Glycine max).

Publisher: Springer Country of Publication: Netherlands NLM ID: 0372625 Publication Model: Electronic Cited Medium: Internet ISSN: 1572-9699 (Electronic) Linking ISSN: 00036072 NLM ISO Abbreviation: Antonie Van Leeuwenhoek Subsets: MEDLINE

Publication: 2004- : Berlin : Springer
Original Publication: Wageningen, Netherland [etc.] Veenman [etc.]

Glycine max*/microbiology ; Phylogeny* ; RNA, Ribosomal, 16S*/genetics ; Plant Roots*/microbiology ; DNA, Bacterial*/genetics ; Fatty Acids*/analysis; Bacterial Typing Techniques ; Sequence Analysis, DNA ; Nucleic Acid Hybridization ; Base Composition

A Gram-staining-positive, aerobic bacterium, designated strain JJ-181  ^T , was isolated from the root surface of soybean. Based on the 16S rRNA gene sequence similarities, strain JJ-181  ^T was grouped into the genus Cohnella, most closely related to Cohnella hashimotonis F6_2S_P_1 ^T (98.85%) and C. ginsengisoli GR21-5  ^T (98.3%). The pairwise average nucleotide identity and digital DNA-DNA hybridisation values of the JJ-181  ^T genome assembly against publicly available Cohnella type strain genomes were below 84% and 28%, respectively. The fatty acid profile from whole cell hydrolysates, the cell wall diaminoacid, the quinone system, the polar lipid profile, and the polyamine pattern supported the allocation of strain JJ-181  ^T to the genus Cohnella. In addition, the results of physiological and biochemical tests also allowed phenotypic differentiation of strain JJ-181  ^T from its closely-related Cohnella species. Functional analysis revealed that strain JJ-181  ^T has different gene clusters related to swarming motility, chemotaxis ability, and endospore and biofilm formations. The gene content also suggests the ability of strain JJ-181  ^T to synthesise biotin and riboflavin, as well as indole-3-acetic acid, an important phytohormone for plant growth. Based on polyphasic analyses, strain JJ-181  ^T can be classified as a new species of the genus Cohnella, for which we propose the name Cohnella rhizoplanae sp. nov., with strain JJ-181  ^T (= LMG 31678  ^T  = CIP 112018  ^T  = CCM 9031  ^T  = DSM 110650  ^T ) as the type strain.
Competing Interests: Declarations. Competing interests: The authors declare no competing interests. Ethics approval: This article does not contain any studies with human participants or animals performed by any of the authors.
(© 2024. The Author(s).)

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Keywords: Cohnella; Genomics; Phylogenetics; Taxonomy

0 (RNA, Ribosomal, 16S)
0 (DNA, Bacterial)
0 (Fatty Acids)

Date Created: 20241224 Date Completed: 20241224 Latest Revision: 20241224

20241224

10.1007/s10482-024-02051-y

39718652