Identification and analysis of drought-responsive F-box genes in upland rice and involvement of OsFBX148 in ABA response and ROS accumulation.

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  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 100967807 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2229 (Electronic) Linking ISSN: 14712229 NLM ISO Abbreviation: BMC Plant Biol Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : BioMed Central, [2001-
    • Subject Terms:
      Oryza*/genetics ; Oryza*/metabolism ; Oryza*/physiology ; F-Box Proteins*/genetics ; F-Box Proteins*/metabolism ; Droughts* ; Plant Proteins*/genetics ; Plant Proteins*/metabolism ; Abscisic Acid*/metabolism ; Abscisic Acid*/pharmacology ; Reactive Oxygen Species*/metabolism ; Gene Expression Regulation, Plant*; Genes, Plant ; Stress, Physiological/genetics ; Plant Growth Regulators/metabolism ; Phylogeny
    • Abstract:
      Background: Upland rice varieties exhibit significant genetic diversity and broad environmental adaptability, making them ideal candidates for identifying consistently expressed stress-responsive genes. F-box proteins typically function as part of the SKP1-CUL1-F-box protein (SCF) ubiquitin ligase complexes to precisely regulate gene expression and protein level, playing essential roles in the modulation of abiotic stress responses. Therefore, utilizing upland rice varieties for screening stress-responsive F-box genes is a highly advantageous approach.
      Results: Through mRNA-seq analysis in the Brazilian upland rice (cv. IAPAR9), the research identified 29 drought-responsive F-box genes. Gene distribution and duplication analysis revealed these genes are distributed on 11 of the 12 chromosomes and 10 collinear gene pairs were identified on different chromosomes. 13 cis-elements or binding sites were identified in the promoters of the 29 drought-responsive F-box genes by analysis. Protein domain, stability and subcellular localization analysis results suggest that these F-box proteins possess F-box domain and several other domains, and they are mostly unstable proteins with subcellular localization in cytoplasm, nucleus, chloroplasts, mitochondria and endoplasmic reticulum. Most of drought-responsive F-box genes exhibited expression in various tissues such as root, stem, leaf, leaf sheath and panicle except for OsFBO10 and OsFBX283. These genes exhibited various responses to abiotic stresses such as osmotic, cold, heat, and salt stresses, along with ABA treatment. Importantly, a frame-shift mutation in OsFBX148 was created in the ZH11 variety, leading to altered ABA signal transduction and ROS accumulation. The study further elucidated the interaction of OsFBX148 with SKP1 family proteins OSK4/7/17 to form the SCF complex, dependent on the F-box domain.
      Conclusions: The research identified and analyzed 29 drought-responsive F-box genes in upland rice and provides valuable insights into the role of OsFBX148 in ABA and ROS responses. It establishes a basis for future exploration of F-box genes in improving resistance to abiotic stresses, especially drought.
      Competing Interests: Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests.
      (© 2024. The Author(s).)
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    • Grant Information:
      32360075 the National Natural Science Foundation of China; 2022CFB694 the Scientific Research Project of Department of Science and Technology of Hubei Province; SCKJ-JYRC-2023-35 "Yazhou Bay" Elite Talent Science and Technology Program of Sanya Yazhou Bay Science and Technology City; PY22005 the Incubation Project of Hubei Minzu University
    • Contributed Indexing:
      Keywords: ABA response; Abiotic stress; OsFBX148; Transcriptome-wide identification; Upland rice
    • Accession Number:
      0 (F-Box Proteins)
      0 (Plant Proteins)
      72S9A8J5GW (Abscisic Acid)
      0 (Reactive Oxygen Species)
      0 (Plant Growth Regulators)
    • Publication Date:
      Date Created: 20241124 Date Completed: 20241124 Latest Revision: 20241127
    • Publication Date:
      20241202
    • Accession Number:
      PMC11587610
    • Accession Number:
      10.1186/s12870-024-05820-z
    • Accession Number:
      39581968