The Sexual Dimorphism in Rectum and Protein Digestion Pathway Influence Sex Pheromone Synthesis in Male Bactrocera Dorsalis.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
  • Additional Information
    • Source:
      Publisher: WILEY-VCH Country of Publication: Germany NLM ID: 101664569 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2198-3844 (Electronic) Linking ISSN: 21983844 NLM ISO Abbreviation: Adv Sci (Weinh) Subsets: MEDLINE
    • Publication Information:
      Original Publication: Weinheim : WILEY-VCH, [2014]-
    • Subject Terms:
    • Abstract:
      Sexual dimorphism is a crucial aspect of mating and reproduction in many animals, yet the molecular mechanisms remain unclear. In Bactrocera dorsalis, sex pheromones trimethylpyrazine (TMP) and tetramethylpyrazine (TTMP) are specifically synthesized by Bacillus strains in the male rectum. In the female rectum, Bacillus strains are found, but TMP and TTMP are not, indicating sexually dimorphic differences in sex pheromone synthesis. Our anatomical observations and precursor measurements revealed significant differences in rectal structure and ammonium levels between sexes.  In vitro and in vivo experiments reveal that ammonium is vital for sex pheromone synthesis in rectal Bacillus strains. Comparative transcriptome analysis identified ammonium-producing genes (carboxypeptidase B and peptide transporter) in the protein digestion pathway that show much higher expression in the male rectum than in the female rectum. Knocking down the expression of either carboxypeptidase B (or inhibiting enzyme activity) or peptide transporter decreases rectal ammonium levels significantly, resulting in the failure of sex pheromone synthesis in the male rectum. This study provides insights into the presence of sexual dimorphism in internal organs and their functionalities in male-specific sex pheromone synthesis and has significant implications for understanding the molecular mechanisms underlying sex pheromone synthesis by symbionts in insects.
      (© 2024 The Author(s). Advanced Science published by Wiley‐VCH GmbH.)
    • References:
      Sci Rep. 2017 Jun 22;7(1):4063. (PMID: 28642479)
      Nat Commun. 2017 Aug 16;8(1):265. (PMID: 28814724)
      Genome Biol. 2014;15(12):550. (PMID: 25516281)
      Elife. 2023 Jan 19;12:. (PMID: 36656757)
      Nat Prod Rep. 2018 May 25;35(5):386-397. (PMID: 29565067)
      Trends Ecol Evol. 2013 Jun;28(6):325-6. (PMID: 23141109)
      Nat Protoc. 2016 Sep;11(9):1650-67. (PMID: 27560171)
      Nature. 1975 Mar 13;254(5496):136-7. (PMID: 804144)
      Sci Rep. 2017 Jul 20;7(1):6041. (PMID: 28729739)
      Curr Biol. 2020 Nov 16;30(22):R1363-R1365. (PMID: 33202233)
      Proc Natl Acad Sci U S A. 2014 Jan 28;111(4):1343-8. (PMID: 24474758)
      J Exp Biol. 2008 Aug;211(Pt 15):2379-87. (PMID: 18626071)
      Proc Biol Sci. 2017 Oct 25;284(1865):. (PMID: 29046376)
      Nature. 2000 Feb 24;403(6772):851. (PMID: 10706273)
      Trends Ecol Evol. 2008 Apr;23(4):220-8. (PMID: 18308422)
      Curr Opin Insect Sci. 2021 Oct;47:25-30. (PMID: 33609767)
      J Chem Ecol. 2022 Aug;48(7-8):598-608. (PMID: 35397045)
      Biochim Biophys Acta. 2006 Mar-Apr;1759(3-4):177-85. (PMID: 16675043)
      Mol Biol Evol. 2018 Feb 1;35(2):465-472. (PMID: 29165628)
      Annu Rev Physiol. 2004;66:361-84. (PMID: 14977407)
      Cell. 2012 Dec 7;151(6):1345-57. (PMID: 23217715)
      Biochimie. 2014 Dec;107 Pt A:15-21. (PMID: 25066916)
      Protein Sci. 2021 Dec;30(12):2445-2456. (PMID: 34658092)
      Science. 2011 Jan 7;331(6013):73-5. (PMID: 21212355)
      Proc Natl Acad Sci U S A. 2015 Dec 22;112(51):15678-83. (PMID: 26644557)
      Evolution. 2015 Nov;69(11):2862-75. (PMID: 26420758)
      Genetics. 2020 Feb;214(2):235-264. (PMID: 32029579)
      J Exp Biol. 2019 Mar 1;222(Pt 5):. (PMID: 30679242)
      Nature. 1968 Aug 10;219(5154):631-2. (PMID: 5665720)
      Ecol Evol. 2012 Jan;2(1):227-46. (PMID: 22408739)
      Metabolomics. 2019 Jan 22;15(2):16. (PMID: 30830445)
      Bioprocess Biosyst Eng. 2010 Oct;33(8):953-9. (PMID: 20306320)
      Adv Sci (Weinh). 2024 Nov;11(44):e2407353. (PMID: 39377305)
      Appl Biochem Biotechnol. 2013 Feb;169(4):1346-52. (PMID: 23306895)
      Insects. 2021 Jan 08;12(1):. (PMID: 33430009)
      Microsc Res Tech. 2005 Aug 1;67(5):219-26. (PMID: 16170821)
      J Chem Ecol. 2013 Mar;39(3):358-63. (PMID: 23397457)
      J Appl Microbiol. 2002;92(4):759-63. (PMID: 11966918)
      J Exp Zool B Mol Dev Evol. 2015 Jan;324(1):68-76. (PMID: 25504930)
      Dig Dis Sci. 2007 Jan;52(1):1-17. (PMID: 17205399)
      J Ind Microbiol Biotechnol. 2010 Aug;37(8):815-21. (PMID: 20437078)
      Nat Methods. 2015 Apr;12(4):357-60. (PMID: 25751142)
      Nature. 2007 May 10;447(7141):202-5. (PMID: 17495925)
      Z Naturforsch C J Biosci. 2017 Sep 26;72(9-10):387-403. (PMID: 28742527)
      Curr Opin Genet Dev. 2021 Aug;69:129-139. (PMID: 33848958)
      Nat Methods. 2006 Oct;3(10):833-8. (PMID: 16964256)
      Curr Biol. 2021 May 24;31(10):2220-2226.e4. (PMID: 33740424)
      Sci China Life Sci. 2015 Feb;58(2):124-36. (PMID: 25563981)
      ISME J. 2023 Oct;17(10):1741-1750. (PMID: 37550382)
      J Insect Physiol. 2021 Apr;130:104210. (PMID: 33610542)
      BMC Mol Biol. 2010 Oct 06;11:76. (PMID: 20923571)
      Sci Adv. 2016 Feb 12;2(2):e1500983. (PMID: 26933680)
      Annu Rev Entomol. 2011;56:445-64. (PMID: 20822452)
    • Grant Information:
      32372520 National Natural Science Foundation of China; 3212200346 National Natural Science Foundation of China; 2023YFD1401401-08 National Key Research and Development Program of China
    • Contributed Indexing:
      Keywords: insect‐microbe interaction; rectum; sex pheromone; sexual dimorphism
    • Accession Number:
      0 (Sex Attractants)
    • Subject Terms:
      Bactrocera dorsalis
    • Publication Date:
      Date Created: 20241008 Date Completed: 20241127 Latest Revision: 20241130
    • Publication Date:
      20241202
    • Accession Number:
      PMC11600207
    • Accession Number:
      10.1002/advs.202407353
    • Accession Number:
      39377305