Molecular and serological prevalence rates of Neospora caninum infection in dogs from Jordan.

Publisher: Springer International Country of Publication: Germany NLM ID: 8703571 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-1955 (Electronic) Linking ISSN: 09320113 NLM ISO Abbreviation: Parasitol Res Subsets: MEDLINE

Original Publication: Berlin : Springer International, c1987-

Neospora*/genetics ; Neospora*/immunology ; Neospora*/isolation & purification ; Dog Diseases*/epidemiology ; Dog Diseases*/parasitology ; Coccidiosis*/epidemiology ; Coccidiosis*/veterinary ; Coccidiosis*/parasitology ; Feces*/parasitology; Animals ; Dogs ; Seroepidemiologic Studies ; Jordan/epidemiology ; Cross-Sectional Studies ; Female ; Male ; Prevalence ; Antibodies, Protozoan/blood ; Polymerase Chain Reaction/veterinary ; Fluorescent Antibody Technique, Indirect/veterinary

Neosporosis is a proven disease of farm animals and dogs caused by Neospora caninum. This cross-sectional study investigates N. caninum prevalence and seroprevalence among 268 dogs. Nc5 gene PCR was carried out on dog faeces and confirmed by sequencing. Seroprevalence was detected using an indirect fluorescent antibody test (IFAT). Three age groups, gender, locality (Amman, Irbid, and Zarqa Governorates), dog type (stray, pet, and breeding), place of living (indoor/outdoor), food type (raw/cooked), having diarrhoea, having abortion in the area, and having animals nearby were tested as independent variables for associations with positivity to N. caninum using univariate and multivariable logistic regression analyses. The true prevalence of N. caninum was 34.3% (95% CI 28.4, 40.5) using the Nc5-PCR test. The true seroprevalence rate of N. caninum among dogs in Jordan was 47.9% (95% CI 41.4, 54.5) using IFAT. The sequenced isolates of Nc5-PCR products (n = 85) matched three N. caninum strains, namely, NcHareGre (n = 70, 82.4%, 95% CI 72.6-89), NC MS2 (n = 14, 16.5%, 95% CI 9.3-26.1), and L218 (n = 1, 1.2%, 95% CI 0.03-6.4). The three strains were isolated previously from three different countries and continents. N. caninum shedding is associated with abortion among dogs and animals in the area (odds ratio = 3.6). In Amman and Zarqa, living indoors reduced seroprevalence at 0.45, 0.24, and 0.02 odds ratios, respectively. Jordan shares three molecular N. caninum strains with three different countries and continents.
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215/2021 Deanship of Research, Jordan University of Science and Technology; 215/2021 Deanship of Research, Jordan University of Science and Technology; 215/2021 Deanship of Research, Jordan University of Science and Technology

Keywords: Neospora caninum; Dogs; IFAT; Jordan; PCR; Prevalence

0 (Antibodies, Protozoan)

Date Created: 20240803 Date Completed: 20240803 Latest Revision: 20240905

20240905

10.1007/s00436-024-08296-w

39096359