Unveiling the role of hydroxyapatite and hydroxyapatite/silver composite in osteoblast-like cell mineralization: An exploration through their viscoelastic properties.

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  • Additional Information
    • Source:
      Publisher: Elsevier Science Country of Publication: United States NLM ID: 8504048 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2763 (Electronic) Linking ISSN: 18732763 NLM ISO Abbreviation: Bone Subsets: MEDLINE
    • Publication Information:
      Publication: New York : Elsevier Science
      Original Publication: Elmsford, NY : Pergamon Press, c1985-
    • Subject Terms:
      Durapatite*/chemistry ; Osteoblasts*/metabolism ; Osteoblasts*/drug effects ; Osteoblasts*/cytology ; Silver*/chemistry ; Calcification, Physiologic*/physiology ; Calcification, Physiologic*/drug effects ; Elasticity*; Viscosity ; Cell Line ; Humans ; Microscopy, Atomic Force ; Animals
    • Abstract:
      Mechanical properties are becoming fundamental for advancing the comprehension of cellular processes. This study addresses the relationship between viscoelastic properties and the cellular mineralization process. Osteoblast-like cells treated with an osteogenic medium were employed for this purpose. Additionally, the study explores the impact of hydroxyapatite (HA) and hydroxyapatite/silver (HA/Ag) composite on this process. AFM relaxation experiments were conducted to extract viscoelastic parameters using the Fractional Zener (FZ) and Fractional Kelvin (FK) models. Our findings revealed that the main phases of mineralization are associated with alterations in the viscoelastic properties of osteoblast-like cells. Furthermore, HA and HA/Ag treatments significantly influenced changes in the viscoelastic properties of these cells. In particular, the HA/Ag treatment demonstrated a marked enhancement in cell fluidity, suggesting a possible role of silver in accelerating the mineralization process. Moreover, the study underscores the independence observed between fluidity and stiffness, indicating that modifications in one parameter may not necessarily correspond to changes in the other. These findings shed light on the factors involved in the cellular mineralization process and emphasize the importance of using viscoelastic properties to discern the impact of treatments on cells.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024 Elsevier Inc. All rights reserved.)
    • Contributed Indexing:
      Keywords: Hydroxyapatite particles; Hydroxyapatite/silver particles; MG-63 cells; Mineralization process; Viscoelasticity
    • Publication Date:
      Date Created: 20240405 Date Completed: 20240518 Latest Revision: 20240518
    • Publication Date:
      20240519
    • Accession Number:
      10.1016/j.bone.2024.117090
    • Accession Number:
      38579924