Physiological and biochemical responses of selected weed and crop species to the plant-based bioherbicide WeedLock.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Superoxide Dismutase*/metabolism ; Photosynthesis*; Catalase/metabolism ; Chlorophyll/pharmacology ; Malondialdehyde ; Antioxidants/pharmacology ; Antioxidants/metabolism ; Stress, Physiological ; Plant Leaves/metabolism

WeedLock is a broad-spectrum plant-based bioherbicide that is currently on the market as a ready-to-use formulation. In this study, we investigated the physiological and biochemical effects of WeedLock (672.75 L ha ^-1 ) on Ageratum conyzoides L., Eleusine indica (L.) Gaertn, Zea mays L., and Amaranthus gangeticus L. at four different time points. WeedLock caused significant reductions in chlorophyll pigment content and disrupted photosynthetic processes in all test plants. The greatest inhibition in photosynthesis was recorded in A. conyzoides at 24 h post-treatment with a 74.88% inhibition. Plants treated with WeedLock showed increased malondialdehyde (MDA) and proline production, which is indicative of phytotoxic stress. Remarkably, MDA contents of all treated plants increased by more than 100% in comparison to untreated. The activity of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) was elevated following treatment with WeedLock. Significant increases were observed in the SOD activity of A. conyzoides ranging from 69.66 to 118.24% from 6 to 72 h post-treatment. Our findings confirm that WeedLock disrupts the normal physiological and biochemical processes in plants following exposure and that its mode of action is associated with ROS (reactive oxygen species) production, similar to that of PPO (protoporphyrinogen oxidase) inhibitors, although specific site-of-action of this novel bioherbicide warrants further investigation.
(© 2022. The Author(s).)

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UPM/800-3/3/1/MATCHING/2018/9300451 Universiti Putra Malaysia; MATCHING/6300931-10201 EntoGenex Industries Sdn. Bhd., Malaysia

EC 1.11.1.6 (Catalase)
EC 1.15.1.1 (Superoxide Dismutase)
1406-65-1 (Chlorophyll)
4Y8F71G49Q (Malondialdehyde)
0 (Antioxidants)

Date Created: 20221115 Date Completed: 20221118 Latest Revision: 20230109

20240829

PMC9666524

10.1038/s41598-022-24144-2

36379972