Peroxisome proliferator-activated receptor gamma regulates genes involved in milk fat synthesis in mammary epithelial cells of water buffalo.

Publisher: Wiley Country of Publication: Australia NLM ID: 100956805 Publication Model: Print Cited Medium: Internet ISSN: 1740-0929 (Electronic) Linking ISSN: 13443941 NLM ISO Abbreviation: Anim Sci J Subsets: MEDLINE

Publication: Richmond, Vic. : Wiley
Original Publication: Tokyo, Japan : Japanese Society of Zootechnical Science [1999-

Buffaloes/*genetics ; Buffaloes/*metabolism ; Epithelial Cells/*metabolism ; Gene Expression/*genetics ; Gene Expression Regulation/*genetics ; Glycolipids/*metabolism ; Glycoproteins/*metabolism ; Lipid Droplets/*metabolism ; Mammary Glands, Animal/*cytology ; Milk/*metabolism ; PPAR gamma/*genetics ; PPAR gamma/*physiology; Animals ; CD36 Antigens/genetics ; CD36 Antigens/metabolism ; Fatty Acid Binding Protein 3/genetics ; Fatty Acid Binding Protein 3/metabolism ; Female ; Intracellular Signaling Peptides and Proteins/genetics ; Intracellular Signaling Peptides and Proteins/metabolism ; Triglycerides/metabolism

Peroxisome proliferator-activated receptor gamma (PPARγ) is a critical transcription factor regulating lipid and glucose metabolism. However, the regulatory effect of PPARγ on milk fat synthesis in buffalo mammary gland is not clear. In order to explore the role of buffalo PPARG gene in milk fat synthesis, lentivirus-mediated interference was used to knock it down and then the recombinant fusion expression vector was transfected into buffalo mammary epithelial cell (BMEC) to overexpress it. PPARG gene knockdown significantly decreased the expression of CD36, FABP3, FABP4, ACSS2, ELOVL6, DGAT2, BTN1A1, AGPAT6, LPIN1, ABCG2, PPARGC1A, INSIG1, FASN, and SREBF2 genes and significantly upregulated the expression of INSIG2 gene but had no significant effect on the expression of ACSL1, GPAM, and SREBF1 genes. PPARG overexpression significantly increased the relative mRNA abundance of CD36, FABP3, FABP4, ACSS2, ELOVL6, DGAT2, BTN1A1, AGPAT6, LPIN1, PPARGC1A, INSIG1, and SREBF2 genes and significantly downregulated the expression of INSIG2 gene but had no significant effect on the expression of ACSL1, GPAM, ABCG2, FASN, and SREBF1 genes. In addition, knockdown/overexpression of PPARG gene significantly decreased/increased triacylglycerol (TAG) content in BMECs. This study revealed that buffalo PPARG gene is a key gene regulating buffalo milk fat synthesis.
(© 2021 Japanese Society of Animal Science.)

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2007C0003Z Natural Science Foundation Key Project of Yunnan Province, China; 2014FA032 Natural Science Foundation Key Project of Yunnan Province, China; 31460582 National Natural Science Foundation of China; 31760659 National Natural Science Foundation of China

Keywords: PPARG; knockdown; milk fat synthesis; overexpression; water buffalo

0 (CD36 Antigens)
0 (Fatty Acid Binding Protein 3)
0 (Glycolipids)
0 (Glycoproteins)
0 (Intracellular Signaling Peptides and Proteins)
0 (PPAR gamma)
0 (Triglycerides)
0 (milk fat globule)

Date Created: 20210308 Date Completed: 20210910 Latest Revision: 20230328

20240829

10.1111/asj.13537

33682250