Protease-resistant cell meshworks: An indication of membrane nanotube-based syncytia formation.

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  • Additional Information
    • Source:
      Publisher: Academic Press Country of Publication: United States NLM ID: 0373226 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2422 (Electronic) Linking ISSN: 00144827 NLM ISO Abbreviation: Exp Cell Res Subsets: MEDLINE
    • Publication Information:
      Publication: Orlando Fl : Academic Press
      Original Publication: New York, Academic Press.
    • Subject Terms:
      Actin Cytoskeleton/*genetics ; Cell Communication/*genetics ; Giant Cells/*physiology ; Peptide Hydrolases/*chemistry; Actin Cytoskeleton/physiology ; Actins/genetics ; Actins/physiology ; Animals ; Cell Communication/physiology ; Cell Membrane Structures/genetics ; Cell Membrane Structures/physiology ; Fibroblasts/physiology ; Gap Junctions/genetics ; Gap Junctions/physiology ; Morphogenesis/genetics ; Morphogenesis/physiology ; Nanotubes/chemistry ; Rats ; Synapses/genetics ; Synapses/physiology
    • Abstract:
      Cell biology considers most animal tissues as assemblies of "individual" cells that rely on different contact-dependent communication mechanisms, including synapses, gap junctions or - a recent awareness - membrane nano- and microtubes. However, by protease-mediated singularization of dense 2D/ 3D cell cultures and tissue explants, we show here that cell collectives stay connected via a continuous meshwork of F-actin-based membrane tubes, resembling tunneling nanotube (TNT)-based networks observed between dispersed cell cultures. Fusion of respective tubes was accompanied by the ingrowth of microtubules and the invasion of mitochondria and lysosomes. Remarkably, in homology to the plasmodesmata-based plant symplast, we found evidence for expanded, membrane-based syncytia in animal tissues by observing dye transfer among the highly interlinked cells. This approach allows for the first time to visualize and quantify membrane continuity-based connections among densely packed cells and to assess their potential physiological and pathological impact closer to the in vivo situation.
      (Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.)
    • Accession Number:
      0 (Actins)
      EC 3.4.- (Peptide Hydrolases)
    • Publication Date:
      Date Created: 20180925 Date Completed: 20190920 Latest Revision: 20190920
    • Publication Date:
      20240829
    • Accession Number:
      10.1016/j.yexcr.2018.09.012
    • Accession Number:
      30248328