A new Luminex-based peptide assay to identify reactivity to baked, fermented, and whole milk.

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  • Additional Information
    • Source:
      Publisher: Wiley-Blackwell Country of Publication: Denmark NLM ID: 7804028 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1398-9995 (Electronic) Linking ISSN: 01054538 NLM ISO Abbreviation: Allergy Subsets: MEDLINE
    • Publication Information:
      Publication: Copenhagen : Wiley-Blackwell
      Original Publication: Copenhagen, Munksgaard.
    • Subject Terms:
      Peptides*; Allergens/*immunology ; Cultured Milk Products/*adverse effects ; Luminescent Measurements/*methods ; Milk/*adverse effects ; Milk Hypersensitivity/*diagnosis ; Milk Hypersensitivity/*immunology; Animals ; Female ; Humans ; Immunoassay/methods ; Immunoglobulin E/blood ; Immunoglobulin E/immunology ; Immunoglobulin G/immunology ; Male ; Protein Binding ; Sensitivity and Specificity
    • Abstract:
      Background: The majority of children with cow's milk allergy (CMA) tolerate baked milk. However, reactivity to fermented milk products such as yogurt/cheese has not been previously evaluated. We sought to determine whether children with CMA could tolerate yogurt/cheese and whether a patient's IgE and IgG4-binding pattern to milk protein epitopes could distinguish clinical reactivity.
      Methods: Four groups of reactivity were identified by Oral food challenge: baked milk reactive, fermented milk reactive, whole milk reactive, and outgrown. sIgE and sIgG4 binding to milk protein epitopes were assessed with a novel Luminex-based peptide assay (LPA). Using machine learning techniques, a model was developed to predict different degrees of CMA.
      Results: The baked milk reactive patients demonstrated the highest degree of IgE epitope binding, which was followed sequentially by fermented milk reactive, whole milk reactive, and outgrown. Data were randomly divided into two groups with 75% of the data utilized for model development (n = 68) and 25% for testing (n = 21). All 68 children used for training were correctly classified with models using IgE and IgG4 epitopes. The average cross-validation accuracy was much higher for models using IgE plus IgG4 epitopes by LPA (84.8%), twice the performance of the serum component proteins assayed by UniCAP (41.9%). The performance of the model on "unseen data" was tested using the 21 withheld patients, and the accuracy of IgE was 86% (AUC = 0.89) while of IgE+IgG4 model was 81% (AUC = 0.94).
      Conclusion: Using a novel high-throughput LPA, we were able to distinguish the diversity of IgE/IgG4 binding to epitopes in the varying CMA phenotypes. LPA is a promising tool to predict correctly different degrees of CMA.
      (© 2018 EAACI and John Wiley and Sons A/S. Published by John Wiley and Sons Ltd.)
    • Grant Information:
      International David H. and Julia Koch Research Program in Food Allergy Therapeutics.
    • Contributed Indexing:
      Keywords: IgE; IgG4; baked milk; epitope; fermented milk
    • Accession Number:
      0 (Allergens)
      0 (Immunoglobulin G)
      0 (Peptides)
      37341-29-0 (Immunoglobulin E)
    • Publication Date:
      Date Created: 20180731 Date Completed: 20200313 Latest Revision: 20200313
    • Publication Date:
      20240829
    • Accession Number:
      10.1111/all.13581
    • Accession Number:
      30058196