[Identification of common medicinal snakes in medicated liquor of Guangdong by COI barcode sequence].

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  • Author(s): Liao J; Chao Z; Zhang L
  • Source:
    Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials [Zhong Yao Cai] 2013 Nov; Vol. 36 (11), pp. 1740-2.
  • Publication Type:
    English Abstract; Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    Chinese
  • Additional Information
    • Source:
      Publisher: Guo jia yi yao guan li ju Zhong yao cai qing bao zhong xin zhan Country of Publication: China NLM ID: 9426370 Publication Model: Print Cited Medium: Print ISSN: 1001-4454 (Print) Linking ISSN: 10014454 NLM ISO Abbreviation: Zhong Yao Cai Subsets: MEDLINE
    • Publication Information:
      Publication: Guangzhou Shi : Guo jia yi yao guan li ju Zhong yao cai qing bao zhong xin zhan
      Original Publication: Guangzhou Shi : Guo jia yi yao guan li ju Zhong yao cai qing bao zhong xin zhan,
    • Subject Terms:
      DNA Barcoding, Taxonomic* ; Materia Medica* ; Phylogeny*; Electron Transport Complex IV/*genetics ; Snakes/*genetics; Animals ; Base Sequence ; China ; DNA Primers ; DNA, Ribosomal Spacer ; Molecular Sequence Data ; Polymerase Chain Reaction ; Sequence Analysis, DNA ; Snakes/classification ; Species Specificity ; Wine
    • Abstract:
      Objective: To identify the common snakes in medicated liquor of Guangdong using COI barcode sequence,and to test the feasibility.
      Methods: The COI barcode sequences of collected medicinal snakes were amplified and sequenced. The sequences combined with the data from GenBank were analyzed for divergence and building a neighbor-joining(NJ) tree with MEGA 5.0.
      Results: The genetic distance and NJ tree demonstrated that there were 241 variable sites in these species, and the average (A + T) content of 56.2% was higher than the average (G + C) content of 43.7%. The maximum interspecific genetic distance was 0.2568, and the minimum was 0. 1519. In the NJ tree,each species formed a monophyletic clade with bootstrap supports of 100%.
      Conclusion: DNA barcoding identification method based on the COI sequence is accurate and can be applied to identify the common medicinal snakes.
    • Accession Number:
      0 (DNA Primers)
      0 (DNA, Ribosomal Spacer)
      0 (Materia Medica)
      EC 1.9.3.1 (Electron Transport Complex IV)
    • Publication Date:
      Date Created: 20140625 Date Completed: 20140904 Latest Revision: 20140624
    • Publication Date:
      20221213
    • Accession Number:
      24956810